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Mycobacterium smegmatis IL-17A and preparation method thereof

A technology of Mycobacterium smegmatis and IL-17A, which is applied in the field of recombinant Mycobacterium smegmatis and its preparation, has achieved good application prospects

Active Publication Date: 2012-07-18
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the recombinant Mycobacterium smegmatis expressing IL-17A (Interleukin-17A, Interleukin 17A) has not been reported yet.

Method used

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  • Mycobacterium smegmatis IL-17A and preparation method thereof
  • Mycobacterium smegmatis IL-17A and preparation method thereof
  • Mycobacterium smegmatis IL-17A and preparation method thereof

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Embodiment Construction

[0027] The present invention provides a Mycobacterium smegmatis strain IL-17A expressing recombinant interleukin-17A, which is different from the existing Mycobacterium smegmatis in that the Escherichia coli-mycobacterium shuttle expression vector expressing the IL-17A gene is in the Expressed in Mycobacterium smegmatis.

[0028] Its preparation method is as follows:

[0029] Step 1: Design amplification primers based on the known IL-17A gene sequence, and introduce them into the upstream and downstream primers respectively Bam HI restriction site and Hin d III restriction site; obtain the IL-17A gene expression fragment by PCR reaction; clone the IL-17A gene expression fragment into a vector (such as Escherichia coli-mycobacterium shuttle expression vector, pMFA41 vector) to obtain a recombinant vector; Wherein, the acquisition and purification of the IL-17A gene sequence can be operated according to the existing technology, and the operating conditions are introduced in t...

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Abstract

The invention relates to a mycobacterium smegmatis IL-17A and a preparation method thereof. An escherichia coli-mycobacteria shuttle expression vector for expressing an IL-17A gene is expressed in the mycobacterium smegmatis IL-17A; and a bacterial strain is preserved at Ordinary Microorganism Center of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 4446. The mycobacterium smegmatis IL-17A has the double effects of mycobacterium smegmatis and IL-17A, so that the effects of the recombined mycobacteria on promoting the nonspecific anti-infections immunity and restraining the allergen mediated airway immune inflammatory damage are remarkably enhanced, and particularly, the recombined mycobacteria can play important roles in preventing and controlling the asthma airway injury caused by allergic asthma or respiratory tract infection.

Description

technical field [0001] The invention relates to a Mycobacterium smegmatis (Mycobacterium smegmatis) strain and a preparation method thereof, in particular to a recombinant Mycobacterium smegmatis expressing IL-17A and a preparation method thereof. Background technique [0002] Immune adjuvant, also known as non-specific immune proliferator, is an auxiliary substance that is injected into the body at the same time or in advance with the antigen, which can enhance the body's immune response or change the type of immune response. It can increase the surface area of ​​the antigen and make the antigen easy to be absorbed by macrophages. Phagocytosis, changing the physical properties of antigens, prolonging the action time of antigens, enhancing the role of helper T cells, stimulating the division of sensitized lymphocytes and the production of plasma cells, improving the titration of antibodies in the body's primary and secondary immune responses, and changing the types and types ...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/09A61K35/74A61P37/02A61P37/08A61P11/06C12R1/34A61K39/39
Inventor 张建华吴良霞郭盛范小勇陈凌郝春莉
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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