Application of agaro-oligosaccharide in porphyra haitanensis immunopotentiator and application method thereof

An immunopotentiator and agar oligosaccharide technology, applied in the application field of agar oligosaccharide, can solve the problems such as the research and application of agar oligosaccharide that have not been carried out, and achieve the effects of reducing the number of pathogenic bacteria, widening the efficacy and reducing losses.

Active Publication Date: 2012-07-25
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent (ZL200710031733.1) discloses a new agar oligosaccharide as an immune enhancer for aquaculture animals, but the research and application of agar oligosaccharides on altar laver have not been carried out.

Method used

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  • Application of agaro-oligosaccharide in porphyra haitanensis immunopotentiator and application method thereof
  • Application of agaro-oligosaccharide in porphyra haitanensis immunopotentiator and application method thereof
  • Application of agaro-oligosaccharide in porphyra haitanensis immunopotentiator and application method thereof

Examples

Experimental program
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preparation Embodiment 1

[0027] Make a 1.5% solution of commercially available agar gel, add HCl with a final concentration of 0.5 mol / l, place in a water bath at 50°C, stir and react for 2 hours, neutralize, add the solution to an activated carbon column for adsorption, and first elute with water to remove untreated agar. The adsorbate is then eluted with 5% ethanol to remove monosaccharides with a degree of polymerization less than 2, and finally the solution obtained by eluting with 25% ethanol is concentrated to near dryness under reduced pressure, and freeze-dried to obtain agarose oligosaccharides with a degree of polymerization of 2-10.

[0028] The agar oligosaccharide was made into a solution with a concentration of 200 μg / ml in seawater, and the activity was evenly sprayed on the thallus of altar laver seedlings during the drying period of the altar laver cultured in semi-floating net curtains (1 hour before the high tide was immersed in water). Agarose oligosaccharide solution. Can be repea...

preparation Embodiment 2

[0030] Commercially available agar gel was made into a 2% solution, HAc with a final concentration of 0.3 mol / l was added, stirred and reacted for 6 hours in a water bath at 90° C., and neutralized. The subsequent experimental process was the same as that of Preparation Example 1.

[0031] Add agar oligosaccharides to the altar laver culture bottle in the laboratory, so that the final concentration of oligosaccharides is 100 μg / ml, and the altar laver culture density is 7 mg / ml. After 1 hour of treatment, change to ordinary sea water to continue the cultivation.

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Abstract

The invention discloses application of agaro-oligosaccharide in porphyra haitanensis immunopotentiator. The invention also discloses an application method of agaro-oligosaccharide in porphyra haitanensis immunopotentiator. Agaro-oligosaccharide is uniformly mixed with seawater to obtain agaro-oligosaccharide seawater solution with concentration of 50 to 250 Mug/ml stirring, or the agaro-oligosaccharide is uniformly sprinkled onto seawater crystals to obtain the agaro-oligosaccharide seawater solution with the final concentration of agaro- oligosaccharide in the seawater crystals being 50 to 250Mug/ml; and the prepared agaro-oligosaccharide seawater solution is used for sprinkling frond of porphyra haitanensis in an air exposure period which is cultured in a sea area or for soaking the porphyra haitanensis which is cultured in a laboratory, and the treatment time is 0.5 to 2h. Due to the adoption of the application method, weaknesses of the present technology for preventing the diseases and putridness of the porphyra haitanensis can be overcome, a growth status of the porphyra haitanensis can be improved through a method of immunology, and the resistance of the porphyra haitanensis to pathogenic microorganisms and severe environments can be enhanced.

Description

technical field [0001] The invention relates to an application of agar oligosaccharide, and also relates to an application method of the agar oligosaccharide. Background technique [0002] Altar laver is an important cultured red algae in Zhejiang and Fujian of my country, and it is also a kind of red algae whose cell wall uses agar gum as the skeleton. In recent years, due to the artificial cultivation techniques that do not conform to the growth law, the deterioration of the sea area environment, climate reasons, and pathogenic bacteria diseases, rotten seedlings of altar laver have occurred from time to time. In history, large areas of laver disease have rotted many times, such as In 2008 and 2011, there were serious diseases and rots, and the losses of farmers were very serious. Therefore, in addition to seed improvement and rational cultivation, how to enhance the immune ability and disease resistance of cultured Porphyra is the best strategy to reduce the incidence an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P21/00A01G33/02
Inventor 陈海敏严小军骆其君杨锐何山
Owner NINGBO UNIV
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