Reagent supply system for DNA (deoxyribonucleic acid) sequencer and control method

Abstract

The invention discloses a reagent supply system for a DNA (deoxyribonucleic acid) sequencer. The reagent supply system comprises a preparing pipeline, a buffer pipeline, a main pipeline, a first multi-pass reversing valve, a second multi-pass reversing valve, a first peristaltic pump for extracting a sequencing reaction reagent and a second peristaltic pump for extracting buffer liquid, wherein the preparing pipeline, the buffer pipeline and the main pipeline are connected through a tee connector, inlets of the first multi-pass reversing valve are respectively communicated with reagent bottles; an outlet of the first multi-pass reversing valve is connected with the preparing pipeline through the first peristaltic pump; an outlet of the second peristaltic pump is connected with the buffer pipeline; an inlet of the second multi-pass reversing valve is connected with the main pipe, and outlets of the second multi-pass reversing valve are respectively communicated with a liquid inlet of areaction cabin and a waste liquor bucket. The invention also discloses a control method of the reagent supply system. According to the reagent supply system disclosed by the invention, the reagent and buffer liquid can be immediately and accurately supplied to a plurality of reaction cabins, so a plurality of reactions are carried out at the same time, a plurality of samples can be sequenced at the same time, and the sequencing efficiency of the DNA is greatly improved.

Description

technical field [0001] The invention relates to the technical field of DNA sequencing, in particular to a reagent supply system for a DNA sequencer and a control method for the reagent supply system. Background technique [0002] In the field of DNA sequencing technology, pyrosequencing technology (pyrosequencing) is a new type of enzymatic cascade sequencing technology developed by Nyren et al. in 1987. Its repeatability and accuracy performance are comparable to Sanger method DNA sequencing technology. , while the speed is greatly improved. [0003] Pyrosequencing technology is an enzyme cascade chemiluminescent reaction in the same reaction system catalyzed by four enzymes. The principle of pyrosequencing technology is: after the primer anneals to the template DNA, the four enzymes DNA polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and adenosine triphosphate (Apyrase) Under the synergistic effect of the primer, the polymerization...

AI Technical Summary

Problems solved by technology

[0007] The inventor found that the DNA sequencer in the prior art has only one reaction chamber, and one instrument can only perform one test, and the work efficiency is not high

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