Preparation method of antibacterial fermentation liquid of Solidago canadesis endophytic fungi

A technology of endophytic fungi and fermentation broth, applied in the field of microorganisms

Active Publication Date: 2012-10-10
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

At present, there is no research report on the antibacterial activity of secondary metabolites of endophytic fungi from Solidago canadensis against plant pathogens at home and abroad

Method used

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  • Preparation method of antibacterial fermentation liquid of Solidago canadesis endophytic fungi
  • Preparation method of antibacterial fermentation liquid of Solidago canadesis endophytic fungi

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Embodiment

[0033] The endophytic fungus of Solidago canadensis used in this embodiment is named as Chaetomium globosa yt03 ( Chaetomium globosum yt03), preserved in the General Microorganism Center of China Microbial Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 20, 2011, and the preservation number is CGMCC No.5088;

[0034] Chaetomium globosa yt03 ( Chaetomium globosum yt03) strain, which is a natural microbial drug source for fermenting and obtaining new agricultural antibiotic active ingredients against plant diseases.

[0035] The concrete preparation operation steps of the antibacterial fermentation liquid of solidago canadensis endophytic fungus are as follows:

[0036] (1) Activation of strains

[0037] Under sterile conditions (ultra-clean workbench), use an inoculation loop to pick up a few test tube inclined planes to preserve the strain Chaetomium globosa yt03 ( Chaetom...

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Abstract

The invention relates to a preparation method of an antibacterial fermentation liquid of Solidago canadesis endophytic fungi, which comprises the following operation steps: 1. activating strain; 2. carrying out first-stage seed culture; 3. carrying out second-stage seed culture; and 4. carrying out fermentation culture to obtain brown Solidago canadesis endophytic fungus fermentation liquid, filtering the Solidago canadesis endophytic fungus fermentation liquid in aseptic condition to remove mycelia, and then passing a 0.22 mu m filter to obtain the antibacterial fermentation liquid of Solidago canadesis endophytic fungi. According to the invention, the preparation method of antibacterial fermentation liquid of Chaetomium globosum yt03 with broad spectrum antibacterial activity is determined; and antibacterial test finds that the Chaetomium globosum yt03 antibacterial fermentation liquid has relatively significant hypha growth inhibiting action on six tested plant pathogenic fungi.

Description

Technical field [0001] The present invention is a microbiological technology field involving a fermentation training method, which specifically involves the preparation method of antibacterial fermentation liquid of an endogenous fungal fungal fungus in Canada. Background technique [0002] With the development of the economy and the improvement of people's living standards, people are more and more concerned about environmental pollution, ecological balance, and human health.Especially the requirements for food quality are getting higher and higher.No rough vegetables and green food have been recognized by the whole society.It is imperative to develop and use biological pesticides to carry out biological control.At present, microbial pesticides will show their skills in the development of biological control.Because it has obvious advantages: environmental safety, no pollution, no residues, not easy to produce resistance, low production cost, simple fermentation process, and safe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 郭正彦花日茂柏钰操海群吴祥为李学德唐俊
Owner ANHUI AGRICULTURAL UNIVERSITY
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