Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Preparation method of antibacterial fermentation liquid of Solidago canadesis endophytic fungi

A technology of endophytic fungi and fermentation broth, applied in the field of microorganisms

Active Publication Date: 2012-10-10
ANHUI AGRICULTURAL UNIVERSITY
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the antibacterial activity of secondary metabolites of endophytic fungi from Solidago canadensis against plant pathogens at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of antibacterial fermentation liquid of Solidago canadesis endophytic fungi
  • Preparation method of antibacterial fermentation liquid of Solidago canadesis endophytic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] The endophytic fungus of Solidago canadensis used in this embodiment is named as Chaetomium globosa yt03 ( Chaetomium globosum yt03), preserved in the General Microorganism Center of China Microbial Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 20, 2011, and the preservation number is CGMCC No.5088;

[0034] Chaetomium globosa yt03 ( Chaetomium globosum yt03) strain, which is a natural microbial drug source for fermenting and obtaining new agricultural antibiotic active ingredients against plant diseases.

[0035] The concrete preparation operation steps of the antibacterial fermentation liquid of solidago canadensis endophytic fungus are as follows:

[0036] (1) Activation of strains

[0037] Under sterile conditions (ultra-clean workbench), use an inoculation loop to pick up a few test tube inclined planes to preserve the strain Chaetomium globosa yt03 ( Chaetom...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of an antibacterial fermentation liquid of Solidago canadesis endophytic fungi, which comprises the following operation steps: 1. activating strain; 2. carrying out first-stage seed culture; 3. carrying out second-stage seed culture; and 4. carrying out fermentation culture to obtain brown Solidago canadesis endophytic fungus fermentation liquid, filtering the Solidago canadesis endophytic fungus fermentation liquid in aseptic condition to remove mycelia, and then passing a 0.22 mu m filter to obtain the antibacterial fermentation liquid of Solidago canadesis endophytic fungi. According to the invention, the preparation method of antibacterial fermentation liquid of Chaetomium globosum yt03 with broad spectrum antibacterial activity is determined; and antibacterial test finds that the Chaetomium globosum yt03 antibacterial fermentation liquid has relatively significant hypha growth inhibiting action on six tested plant pathogenic fungi.

Description

Technical field [0001] The present invention is a microbiological technology field involving a fermentation training method, which specifically involves the preparation method of antibacterial fermentation liquid of an endogenous fungal fungal fungus in Canada. Background technique [0002] With the development of the economy and the improvement of people's living standards, people are more and more concerned about environmental pollution, ecological balance, and human health.Especially the requirements for food quality are getting higher and higher.No rough vegetables and green food have been recognized by the whole society.It is imperative to develop and use biological pesticides to carry out biological control.At present, microbial pesticides will show their skills in the development of biological control.Because it has obvious advantages: environmental safety, no pollution, no residues, not easy to produce resistance, low production cost, simple fermentation process, and safe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 郭正彦花日茂柏钰操海群吴祥为李学德唐俊
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com