Detection method and application for single nucleotide polymorphism of miR-196a genes associated with lactation of dairy goats

A technology of single nucleotide polymorphism and detection method, which is applied in the application field of dairy goat breeding to achieve the effects of low cost, low cost and high accuracy

Inactive Publication Date: 2013-11-20
NORTHWEST A & F UNIV
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Problems solved by technology

However, the research on miR-196a in dairy goats has not been reported yet

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  • Detection method and application for single nucleotide polymorphism of miR-196a genes associated with lactation of dairy goats
  • Detection method and application for single nucleotide polymorphism of miR-196a genes associated with lactation of dairy goats
  • Detection method and application for single nucleotide polymorphism of miR-196a genes associated with lactation of dairy goats

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Embodiment Construction

[0038] The present invention first designs primers based on the conservative sequence of the miR-196a gene, uses the genomic DNA pools of four goat breeds as templates to perform PCR amplification, and purifies and sequence the PCR products. Then, perform sequencing analysis and sequence comparison to screen out SNP sites; secondly, perform HinfI-RFLP detection of polymorphic sites in the population to be tested; finally, perform population genetics based on the genotypes detected in the population Statistical analysis and correlation analysis of lactation yield, screening out molecular markers closely related to lactation traits of dairy goats. The present invention will be described in detail below, which is an explanation rather than a limitation of the present invention.

[0039] a. Cloning of goat miR-196a gene DNA sequence and detection of its polymorphism

[0040] 1. Collection and processing of goat blood samples

[0041] Take 10 mL of goat blood sample, add 500μL of 0.5mol...

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Abstract

The invention discloses a detection method and application for miR-196a gene single nucleotide polymorphism associated with lactation of dairy goats. The single nucleotide polymorphism of the genes comprises the single nucleotide polymorphism of which the 340th bit is C or T in a dairy goat miR-196a gene sequence showed in SEQ ID No. 1. Association analysis on the RFLP(restricted fragment length polymorphisms) and the lactation yield proves that: HinfI polymorphic sites are obviously associated with the milk yield, and the milk yield of the second birth and the third birth of which the genotypes are CT are obviously higher than those of individuals of which the genotypes are CC and TT, and in additional, the body height of TT individuals is obviously higher than that of individuals of which the genotypes are CC and CT. The detection method provided by the invention can be used for marker assisted selection (MAS) of the lactation properties of the Capra hircus dairy goats, and the population of the dairy goats with good genetic resources can be quickly constructed.

Description

Technical field [0001] The invention belongs to the technical field of milk goat breeding, and relates to the single nucleotide polymorphism of the miR-196a gene related to milk goat milk production and its detection and its application as an auxiliary selection marker in milk goat breeding. Background technique [0002] my country is rich in goat breed resources and has a long history of sheep breeding. The number of sheep in the world is among the highest in the world. There is huge potential for the development of sheep farming in the vast rural areas, pastoral areas and urban suburbs. The goat industry provides a variety of life products such as milk, meat, wool, cashmere and leather for our lives. Among them, the dairy goat industry has small investment, is easy to raise, and has quick results. It is called "the farmer's cow" (Zhao Youzhang 2002). Goat milk has high nutritional value, does not contain allergens, has balanced nutrients, is easily digested and absorbed, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68A01K67/027
Inventor 陈宏李转见郭文娇蓝贤勇黄永震孙加节王璟
Owner NORTHWEST A & F UNIV
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