Detection device and method for stream mode particle

Abstract

The invention discloses a detection device and method for stream mode particle, including a sample preparing container, a sheath liquid preparing container, a flowing chamber, an accomodating container, a first constant current power source and a pressure monitoring device, the sample preparing container is connected to the flowing chamber via a sample conveying pipe, the sheath liquid preparing container is connected to the flowing chamber via a sheath liquid conveying pipe, the outlet of the flowing chamber is connected to a detecting pipe, the sample to be detected is driven by power to be delivered to the flowing chamber via the sample conveying pipe, the first constant current power source is connected to the sheath liquid conveying pipe and drives the sheath liquid to be conveyed to the flowing chamber via the sheath liquid conveying pipe. The sheath liquid is driven by the constant current power source, thus obviating the need of gas source device, reducing cost and facilitating miniaturization; by real time monitoring the pressure of the flowing chamber, regular flow or abnormal flow can be judged, to prevent risk of breakage of the detecting pipe caused by abnormal increase of pressure, and avoid biological pollution.

Description

technical field [0001] The invention relates to a particle detection device and method. Background technique [0002] In the field of testing (such as blood cell testing), sheath flow technology is used in many links. The sheath flow technology is to wrap the particles in the middle of the sheath fluid, so that the particles pass through the detection part in a straight line. Such as figure 1 As shown, the existing particle detection device generally includes a sample preparation pool 1, a waste liquid pool 2, a sample syringe 3, a quantitative pump 4, a constant pressure sheath liquid pool 5, a sample needle 6, a flow chamber 7, and a pipeline for on-off control. The first valve V1, the second valve V2 and the third valve V3, wherein, the sample preparation pool 1 is used to store the sample to be tested, the waste liquid pool 2 is used to collect the waste liquid generated during the detection process, and the sample syringe 3 is used to The sample to be tested is pushe...

AI Technical Summary

Problems solved by technology

[0004] However, this kind of particle detection device has the following disadvantages: 1) a pressure source is used as the power source of the sheath fluid, that is, a constant pressure source is used as the pressure source, so that a complete set of gas source and related components needs to be provided, and the cost of this solution is high and takes up big space

Images