Method for comprehensively extracting tea oil, flavonoids compounds, tea saponin and tea polysaccharide

A technology for comprehensive extraction of flavonoids, applied in the field of flavonoids, comprehensive extraction of tea oil, tea saponin and tea polysaccharide, to achieve the effects of few steps, good extraction effect and low toxicity

Inactive Publication Date: 2013-02-13
伊国群
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the current problem of lack of methods for effectively extracting multiple active ingredients from Camellia oleifera cake in the same process flow, and to provide a method for comprehensively extracting tea oil, flavonoids, and tea saponin from Camellia oleifera cake. and tea polysaccharides, this method effectively avoids the loss of another active ingredient when extracting one active ingredient, and obtains a better extraction effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for comprehensively extracting tea oil, flavonoids, tea saponin and tea polysaccharides, comprising the following steps:

[0035] (1) Weigh 200g of dried camellia oleifera cake, and use supercritical CO 2 Extraction was carried out once to obtain 199.59g Camellia oleifera cake I and supercritical CO 2 The extract was removed to obtain 0.41 g of camellia oil.

[0036] (2) Take 10g of camellia oleifera cake I, and carry out Soxhlet extraction with 80ml of ethyl acetate for 6 hours to obtain 9.93g of camellia oleifera cake II and ethyl acetate extract, concentrate the ethyl acetate extract under reduced pressure, cool and crystallize After filtration, 0.07 g of flavonoid glycosides was obtained.

[0037] (3) Use 80ml of 70% ethanol-water solution to carry out Soxhlet extraction of the above-mentioned camellia oleifera cake II for 6 hours to obtain 9.91 g of camellia oleifera cake III and ethanol-water solution extract, and evaporate the ethanol in the extract so...

Embodiment 2

[0041] A method for comprehensively extracting tea oil, flavonoids, tea saponin and tea polysaccharides, comprising the following steps:

[0042] (1) Weigh 20g of dried camellia oleifera cake, extract 3 times with 150ml of petroleum ether, each extraction time is 2 hours, and obtain 19.24g of camellia oleifera cake I and petroleum ether extract, and concentrate the petroleum ether extract under reduced pressure to obtain Camellia oil 0.76g.

[0043] (2) Use 150ml of ethyl acetate to extract the above-mentioned camellia oleifera cake I 3 times, each extraction time is 2 hours, to obtain 19.09g of camellia oleifera cake II and ethyl acetate extract, concentrate the ethyl acetate extract under reduced pressure, After cooling and crystallization, 0.15 g of flavonoid glycosides were obtained after filtration.

[0044] (3) Use 150ml of 70% ethanol-water solution to extract the above-mentioned camellia oleifera cake Ⅱ for 3 times, each extraction time is 2 hours, to obtain 19.04g of...

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PUM

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Abstract

The invention provides a method for comprehensively extracting tea oil, flavonoids compounds, tea saponin and tea polysaccharide. The method comprises the following steps of: extracting the tea oil in a dry oil-tea cake by using a low-polarity solvent, and extracting the oil-tea cake by using ethyl acetate, an ethanol-water solution and water in turn to obtain flavonoid glycosides compounds, the tea saponin and the tea polysaccharide respectively. According to the method for comprehensively extracting the tea oil, the flavonoids compounds, the tea saponin and the tea polysaccharide, provided by the invention, five effective components in the oil-tea cake can be comprehensively extracted in the same process, and the method has the advantages of fewer steps, low toxicity of the solvent and good extracting effect.

Description

technical field [0001] The invention relates to the technical field of plant extracts, in particular to a method for comprehensively extracting tea oil, flavonoids, tea saponin and tea polysaccharides. Background technique [0002] Camellia oleifera is one of the oldest woody edible vegetable oils in my country and one of the four largest woody oil plants in the world. It is named because its seeds can be used to extract oil (tea oil) for consumption. Camellia oil is rich in unsaturated fatty acids such as oleic acid, linoleic acid, and linolenic acid, with a content as high as 93%. Long-term consumption of camellia oil can reduce the cholesterol content in serum and effectively prevent cardiovascular diseases, comparable to the most popular edible oil in the world - olive oil. [0003] The residue of camellia oleifera seeds after oil extraction is called camellia oleifera cake, which is generally used as fuel, or simply processed as feed. However, studies have shown that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B13/02C07H15/256C07H1/08C07J63/00C07G99/00C08B37/00
CPCY02P20/54Y02W30/74
Inventor 伊国群孟庆华
Owner 伊国群
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