Method for comprehensively extracting tea oil, flavonoids compounds, tea saponin and tea polysaccharide
A technology for comprehensive extraction of flavonoids, applied in the field of flavonoids, comprehensive extraction of tea oil, tea saponin and tea polysaccharide, to achieve the effects of few steps, good extraction effect and low toxicity
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Embodiment 1
[0034] A method for comprehensively extracting tea oil, flavonoids, tea saponin and tea polysaccharides, comprising the following steps:
[0035] (1) Weigh 200g of dried camellia oleifera cake, and use supercritical CO 2 Extraction was carried out once to obtain 199.59g Camellia oleifera cake I and supercritical CO 2 The extract was removed to obtain 0.41 g of camellia oil.
[0036] (2) Take 10g of camellia oleifera cake I, and carry out Soxhlet extraction with 80ml of ethyl acetate for 6 hours to obtain 9.93g of camellia oleifera cake II and ethyl acetate extract, concentrate the ethyl acetate extract under reduced pressure, cool and crystallize After filtration, 0.07 g of flavonoid glycosides was obtained.
[0037] (3) Use 80ml of 70% ethanol-water solution to carry out Soxhlet extraction of the above-mentioned camellia oleifera cake II for 6 hours to obtain 9.91 g of camellia oleifera cake III and ethanol-water solution extract, and evaporate the ethanol in the extract so...
Embodiment 2
[0041] A method for comprehensively extracting tea oil, flavonoids, tea saponin and tea polysaccharides, comprising the following steps:
[0042] (1) Weigh 20g of dried camellia oleifera cake, extract 3 times with 150ml of petroleum ether, each extraction time is 2 hours, and obtain 19.24g of camellia oleifera cake I and petroleum ether extract, and concentrate the petroleum ether extract under reduced pressure to obtain Camellia oil 0.76g.
[0043] (2) Use 150ml of ethyl acetate to extract the above-mentioned camellia oleifera cake I 3 times, each extraction time is 2 hours, to obtain 19.09g of camellia oleifera cake II and ethyl acetate extract, concentrate the ethyl acetate extract under reduced pressure, After cooling and crystallization, 0.15 g of flavonoid glycosides were obtained after filtration.
[0044] (3) Use 150ml of 70% ethanol-water solution to extract the above-mentioned camellia oleifera cake Ⅱ for 3 times, each extraction time is 2 hours, to obtain 19.04g of...
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