Tandem mass spectrometry analysis method performed in ion traps

Abstract

The invention belongs to the technical field of mass analysis, in particular to a tandem mass spectrometry analysis method performed in ion traps. The method comprises ion selective isolation, collision induced dissociation and quality scanning analysis. Dipole excitation voltages with asymmetric wave shapes are imposed on a pair of electrodes to generate dipole direct voltages, so that isolated parent ions obtain energy to be excited, collided with neutral molecules in the ion traps and dissociated, and tandem mass spectrometry analysis is achieved. The tandem mass spectrometry analysis method performed in ion traps has the advantages that additional direct current power supplies are not needed, and the dipole direct voltages can be obtained only through the control of software, and experiment devices and methods can be simplified apparently. Simultaneously, the parent ions are subjected to a non-resonance excited dissociation mode under the action of the dipole direct voltages, and abundant ion fragment information can be given through the non-resonance excitation of collision and dissociation.

Description

technical field [0001] The invention belongs to the technical field of mass analysis, and specifically relates to a method for realizing tandem mass spectrometry analysis in an ion trap mass spectrometer, which realizes ion excitation and collision-induced dissociation by applying a dipole excitation voltage of an asymmetric waveform. Background technique [0002] As a powerful analytical technique, mass spectrometry can achieve qualitative and quantitative analysis of compounds, and is widely used in pharmaceutical analysis, environmental monitoring, national security, forensic science, proteomics and other fields. As we all know, mass spectrometry can characterize and analyze the structure of compounds through tandem mass spectrometry (Tandem MS) analysis. The analysis process of tandem mass spectrometry is as follows: the first stage is isolation. For the ions in the sample to be analyzed, ions with a specific mass-to-charge ratio (m / z) are selected to isolate them, and t...

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Problems solved by technology

However, this method requires an additional DC power supply to provide DC voltage. The dipole DC application method is realized through the modification of the circuit. At the same time, the dipole DC voltage has timing changes and requires precise control, so the hardware part of the instrument Requirements are bound to become more stringent and complex

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