Chitosan nano particle, biological pesticide preparation for controlling beet armyworms and preparation method thereof

A technology of chitosan nanoparticles and beet armyworm, applied in botanical equipment and methods, biocides, animal repellents, etc., can solve the problems of unrealized standardization level, small molecular weight, limitations, etc., and achieve high pest control effect, improvement of stability, effect of standardization of production steps

Active Publication Date: 2015-04-08
GUANGZHOU RIBOBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above reports have successfully prepared pesticide chitosan nanoparticle preparations, their research and development are still in the exploratory stage, and the physical and chemical indicators such as the size, particle size distribution, and drug loading rate of the prepared nanoparticles are unstable, and the standardization level has not been achieved. It is cumbersome and difficult to achieve large-scale production, so it has been limited to laboratory research so far, and has not been transformed into a product preparation circulation market
In addition, the active ingredients of the pesticide chitosan preparations successfully reported so far are mostly existing chemical pesticides with small molecular weight and relatively simple technical operation.

Method used

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  • Chitosan nano particle, biological pesticide preparation for controlling beet armyworms and preparation method thereof
  • Chitosan nano particle, biological pesticide preparation for controlling beet armyworms and preparation method thereof
  • Chitosan nano particle, biological pesticide preparation for controlling beet armyworms and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation with the double-stranded ribonucleic acid sequence of the beet armyworm cadherin gene as the active ingredient.

[0047] (1) Design and synthesis of active ingredients for double-stranded ribonucleic acid of beet armyworm cadherin gene

[0048] GeneBank accession number: HM116243.1

[0049] Si-Cadherin_001: Target gene TGATG AACTC ACATT CGAA

[0050] Sense strand (5'-3'): 5'UGAUG AACUC ACAUU CGAA dTdT3' SEQ ID NO.1

[0051] Antisense strand (3'-5'): 3'dTdT ACUAC UUGAG UGUAA GCUU5' SEQ ID NO.2

[0052] Si-Cadherin_002: ACTGC GTTTA GAAAA CTTT

[0053] Sense strand (5'-3'): 5'ACUGC GUUUA GAAAA CUUU dTdT3' SEQ ID NO.3

[0054] Antisense strand (3'-5'): 3'dTdT UGACG CAAAU CUUUU GAAA5' SEQ ID NO.4

[0055] Si-Cadherin_003: CGAAG CCCTT TCAAC GACC

[0056] Sense strand (5'-3'): 5'CGAAG CCCUU UCAAC GACC dTdT3' SEQ ID NO.5

[0057] Antisense strand (3'...

Embodiment 2

[0077] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation targeting the actin gene double-stranded ribonucleic acid sequence of beet armyworm as the active ingredient.

[0078] (1) Design and synthesis of the original drug components targeting the actin gene of beet armyworm

[0079] GenBank: AY507963.1

[0080] Si-actin_001: ACTTG GCTGG TCGCG ACTTG

[0081] Sense strand (5'-3'): 5'ACUUG GCUGG UCGCG ACUUG dTdT3' SEQ ID NO.7

[0082] Antisense strand (3'-5'): 3'dTdT UGAAC CGACC AGCGC UGAAC5' SEQ ID NO.8

[0083] Si-actin_002: ACAAC TCCAT CATGA AGTGT

[0084] Sense strand (5'-3'): 5'ACAAC UCCAU CAUGA AGUGU dTdT3' SEQ ID NO.9

[0085] Antisense strand (3'-5'): 3'dTdT UGUUG AGGUA GUACU UCACA5' SEQ ID NO.10

[0086] The 3' end of the above sequence is provided with dTdT dangling bases. The double-stranded ribonucleic acid is designed and synthesized according to the above sequence and freeze-dried into th...

Embodiment 3

[0106] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation targeting the double-stranded ribonucleic acid sequence of the cytochrome P450 gene of beet armyworm as the active ingredient.

[0107] (1) Design and synthesis of double-stranded ribonucleic acid technical drug against cytochrome P450 gene of beet armyworm

[0108] GenBank: HQ340240.1

[0109] Si-C_001: TTCCA AAAGA CAGGT TT

[0110] Sense strand (5'-3'): 5'UUCCA AAAGA CAGGU UU dTdT3' SEQ ID NO.11

[0111] Antisense strand (3'-5'): 3'dTdT AAGGT UUUCU GUCCA AA5' SEQ ID NO.12

[0112] Si-C_002: CTCCA CGTTA AGTCC AG

[0113] Sense strand (5'-3'): 5'CUCCA CGUUA AGUCC AG dTdT3' SEQ ID NO.13

[0114] Antisense strand (3'-5'): 3'dTdT GAGGU GCAAU UCAGG UC5' SEQ ID NO.14

[0115] Si-C_003: TCTCC GATTA TGGCC AC

[0116] Sense strand (5'-3'): 5'UCUCC GAUUA UGGCC AC dTdT3' SEQ ID NO.15

[0117] Antisense strand (3'-5'): 3'dTdT AGAGG CUAAU ACCGG UG5' SEQ ...

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Abstract

The invention discloses a chitosan nano particle which comprises the following raw materials in percentage by weight: 0.5-5% of chitosan, 1-10% of cosolvent, 0-5% of antifreezing agent, 0.1-1% of surfactant, 0.1-0.5% of emulsifier, 40-60% of buffer solution and the balance of water. The invention also provides a biological pesticide preparation for controlling beet armyworms and a preparation method thereof. The biological pesticide preparation mainly comprises the following components in percentage by weight: (A) 0.05-15% of active component: a siRNA (small interfering ribonucleic acid) is designed and synthesized in accordance with a beet armyworm high-efficiency killing target gene and is a 15-50nt double-chain RNA; (B) 19.95-55% of solvent; and (C) 30-80% of chitosan nano particle. The chitosan nano particle ensures that the stability and the beet armyworm control effect of the biological pesticide preparation are better.

Description

technical field [0001] The invention belongs to the technical field of agricultural insecticides, and specifically relates to a biological pesticide preparation and a preparation method thereof, in particular to a chitosan nanoparticle, a double-stranded ribonucleic acid-containing biological pesticide preparation for controlling beet armyworm and a preparation method thereof. technical background [0002] Beet armyworm (Spodoptera exigua (Hubner)), also known as cabbage armyworm and corn leaf armyworm, belongs to Lepidoptera family Noctuidae, and is a worldwide polyphagous and gluttonous pest. In addition to harming food crops, beet armyworm also harms vegetables, cotton, hemp, tobacco, alfalfa and various weeds, with 170 kinds of host plants. Among them, cabbage, beets, cabbage, green onions, amaranth, celery, and cauliflower are the most harmful. At present, more than 20 provinces, municipalities and autonomous regions in my country have the occurrence of beet armyworm. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/04A01N43/90A01P7/04
Inventor 张必良
Owner GUANGZHOU RIBOBIO
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