Kit and preparation method for identifying and detecting a variety of sheep Theileria

A technology of Theileria ovis and Theileria ovis is applied in the field of kits and preparations for identification and detection of various types of Theileria ovis, which can solve the problems of rapid detection and identification of field samples, long identification time, and detection time. Long-term problems, to achieve the effect of reducing detection time and cost, low reagent price, and easy operation
CN103146836BActive Publication Date: 2015-08-19LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Publication Date
2015-08-19

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a multiple PCR (Polymerase Chain Reaction) identification kit for detecting and identifying sheep Loeffler theileria, sheep Dyen theileria and sheep Theileria. The kit for identifying and detecting various sheep theileria comprises three pairs of specific primers, which are respectively upstream and downstream primers of sheep Loeffler theileria, upstream and downstream primers of sheep Dyen theileria, and upstream and downstream primers of sheep theileria. In the specific embodiment of the invention, the primers in the kit for identifying and detecting various sheep theileria are respectively SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5 and SEQ ID NO. 6. The kit disclosed by the invention has the advantages of being simple and convenient to operate for detection, low in price, and capable of simultaneously quickly detecting all the domestic sheep theileria types at present.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a kit for the detection and identification of specific parasites. Specifically, the present invention is a multiplex PCR identification kit that can be used for Theileria ovis, Theileria yoshii, and Theileria ovis. Background technique

[0002] Theileria ovis can infect sheep and goats, and has a global distribution. Theileria ovis from different regions may have different biological characteristics and pathogenicity. According to the shape of the worm, the transmission medium, the pathogenicity, the culture characteristics, etc., combined with the differences in the 18S rRNA gene sequence of Theileria ovis, the Theileria ovis is currently divided into six species, namely, Theileria reinhardt, Theileria ovis, and isolated Theileria, Theileria hidden, Theileria lushii, and Theileria yoshii. In my country, the reported pathogens of Theileria in sheep include Theileria yoshii, Theileria rushii and Theileria ovis. The tr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More