Production method for recombinant human granulocyte colony-stimulating factor

A technology of stimulating factor and production method, applied in the field of production of recombinant human granulocyte stimulating factor, can solve the problems of difficulty in controlling ultrasonic power and time, inability to completely release recombinant protein, protein carbonization, etc., and achieve high speed and large processing capacity. , the effect of reducing difficulty
CN103233053BActive Publication Date: 2014-03-05BEIJING FOUR RINGS BIOPHARM
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING FOUR RINGS BIOPHARM
Publication Date
2014-03-05
Patent Text Reader

Abstract

The invention discloses production method for a recombinant human granulocyte colony-stimulating factor. According to the invention, an engineered strain of an escherichia coli expressed recombinant human granulocyte colony-stimulating factor is cultured to obtain an inclusion body, the engineered strain of the escherichia coli expressed recombinant human granulocyte colony-stimulating factor is pKG931 / HB101, a culture method comprises multiplication culture and culture in a fermentation cylinder, an antibiotic-free medium containing yeast and peptone is employed in both culture, a high pressure homogenizer is used for breaking of bacteria, and denaturation, renaturation and chromatography are carried out so as to obtain a high purity G-CSF stock solution. The production method provided by the invention has the advantages of a short production period, high production efficiency, a great production scale, especial suitability for industrial production and reduction in production cost.
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Description

technical field

[0001] The invention relates to the field of biopharmaceuticals, in particular to a production method of recombinant human granulocyte stimulating factor. Background technique

[0002] Recombinant human granulocyte-stimulating factor (granulocyte colony-stimulating factor, G-CSF) refers to specifically acting on neutrophil progenitor cells in the hematopoietic system, promoting their proliferation, differentiation into mature neutrophils, and maintaining the survival of cells and their biological A hematopoietic growth factor that functions as a colony-stimulating factor that stimulates bone marrow cell colony formation. It has obvious curative effect on neutrophil increase during bone marrow transplantation, severe neutropenia caused by cancer chemotherapy, and neutropenia accompanied by aplastic anemia.

[0003] The biological activity of recombinant G-CSF by genetic engineering technology is similar to that of natural one, and it can be produced in large ...

Claims

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