Method for preparing pallidisetin A
A technology for moss blondii and cyanidin, which is applied in the field of preparing cyanidin A, and achieves the effects of high yield, simple operation and large preparation amount
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Embodiment 1
[0016] Get moss goldensells medicinal material and pulverize, control particle diameter to be 60 orders, take by weighing 500g, be 1:10 to add 85% ethanol solution with solid-liquid volume ratio, adopt ultra-high pressure method to extract, extraction pressure is 400MPa, and holding time is 3.5min, repeat 3 times, combine extracts, concentrate under reduced pressure to obtain extract extract, extract 3 times with n-butanol, combine n-butanol extract, concentrate to obtain extract extract, mix extract with a small amount of neutral magnesium oxide Mix the sample, dry it at low temperature, load the sample to a 100-mesh neutral magnesium oxide low-pressure column (column pressure is 6 bar, column diameter-to-height ratio is 1:7), and use petroleum ether-acetone at a volume ratio of 20:1-3:1. Gradient elution, collect each part of the eluate, concentrate and dry the petroleum ether-acetone (3:1) eluate to obtain the crude product. The crude product was separated and purified by p...
Embodiment 2
[0018] Get moss goldensells medicinal material and pulverize, control particle diameter to be 60 orders, take by weighing 500g, be 1:18 to add 85% ethanol solution with solid-liquid volume ratio, adopt ultra-high pressure method to extract, extraction pressure is 400MPa, and holding time is 5min, repeated 3 times, combined extracts, concentrated under reduced pressure to obtain extract extract, extracted 4 times with n-butanol, combined n-butanol extract, concentrated to obtain extract extract, mixed extract with a small amount of neutral magnesium oxide Samples were dried at low temperature, loaded onto a 200-mesh neutral magnesium oxide low-pressure column (column pressure 5 bar, column diameter-to-height ratio 1:12), and a gradient was carried out with petroleum ether-acetone at a volume ratio of 20:1-3:1 For elution, collect each part of the eluate, concentrate and dry the petroleum ether-acetone (3:1) eluate to obtain a crude product. The crude product was separated and p...
Embodiment 3
[0020] Get moss goldensells medicinal material and pulverize, control particle diameter to be 60 orders, take by weighing 500g, be 1:15 to add 95% ethanol solution with solid-liquid volume ratio, adopt ultra-high pressure method to extract, extraction pressure is 400MPa, and holding time is 3 min, repeat 3 times, combine extracts, concentrate under reduced pressure to obtain extract extract, extract 3 times with n-butanol, combine n-butanol extract, concentrate to obtain extract extract, mix extract with a small amount of neutral magnesium oxide Mix the sample, dry at low temperature, load the sample to a 200-mesh neutral magnesium oxide low-pressure column (column pressure is 7.5bar, column diameter-to-height ratio is 1:10), and use petroleum ether-acetone at a volume ratio of 20:1-3:1 Carry out gradient elution, collect each part of the eluate, concentrate and dry the petroleum ether-acetone (3:1) eluate to obtain the crude product. The crude product was separated and purifi...
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