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Method for authenticating Beijing fatty chickens by microsatellite markers

A technology of microsatellite marking and fried chicken, which is applied in the application field of microsatellite marking, can solve the problems of restricting the preservation and development of chicken breeds, and achieve the effect of low detection cost, high accuracy and easy operation

Active Publication Date: 2013-10-30
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] The current method of identifying poultry populations is mainly through chicken breed morphological markers and production performance measurement, which has certain limitations and restricts the preservation and development of local chicken breeds

Method used

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  • Method for authenticating Beijing fatty chickens by microsatellite markers
  • Method for authenticating Beijing fatty chickens by microsatellite markers
  • Method for authenticating Beijing fatty chickens by microsatellite markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 Obtaining of SSR markers of the present invention

[0012] 100 original breeds of Beijing oil chicken and 40 original breeds of 5 reference breeds (Jingxing yellow chicken D2 line, silky silky chicken, Shiqi mixed chicken, Xianju chicken, Camellia chicken) were randomly selected. The Beijing oil chicken breed comes from the National Breed Preservation Farm of Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences. The ratio of male to female is 1:1. Finally, the genomic DNA of 93 Beijing oil chickens and 40 chickens of other chicken breeds were obtained. 20 pairs of chicken-specific microsatellite markers were used for detection, and the polymorphisms of 19 microsatellite loci could not distinguish Beijing Youji chicken from other populations, and only the length polymorphism of LEI0070 marker in Beijing Youji chicken population had Characteristic, which can distinguish the Beijing Youjibao population from oth...

Embodiment 2

[0015] Embodiment 2 Utilizes the method of SSR marker to identify Beijing fried chicken

[0016] 1.1 Groups to be identified

[0017] Randomly select 40-50 chickens to be tested, the ratio of male to female is 1:1, blood is collected from the wing vein, anticoagulated with ACD, and stored at -20°C for later use.

[0018] 1.2 DNA extraction

[0019] The genomic DNA was extracted by the conventional phenol imitation method, dissolved in TE, and the purity and concentration of the DNA were double-checked by agarose gel electrophoresis and ultraviolet spectrophotometry, and then diluted to a concentration of 50ng / μl.

[0020] 1.3 Primers

[0021] According to chicken-specific SSR primers published in NCBI (http: / / www.ncbi.nlm.nih.gov / genome / sts / sts.cgi?uid=279930) (Table 2):

[0022] Table 2 SSR primers involved in the present invention

[0023]

[0024] 1.4 PCR reaction

[0025] PCR amplification was performed in a BioThermocycler. The PCR reaction program was pre-denatu...

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Abstract

The invention provides a method for authenticating Beijing fatty chickens by microsatellite markers. The method comprises the following steps of: performing polymerase chain reaction (PCR) multiplication and sequencing on an LEI0070 microsatellite seat by taking a gene group of a chicken to be authenticated as a template and 5'-TGCGGAGAGCAATTAGTCTGC-3' and 5'-GGAAAACAATCACTGCCTCG-3' as primers, counting the frequencies of allelic genes on the microsatellite seat according to a sequencing result, and if the sum of the frequencies of three allelic genes, namely 201 bp, 205 bp and 207 bp, is over 75 percent, authenticating the chicken to be authenticated as the Beijing fatty chicken. The method has the characteristics of simplicity in operation, low detection cost and high accuracy.

Description

technical field [0001] The invention relates to the application of microsatellite markers, in particular to a method for identifying Beijing fried chicken by using microsatellite markers. Background technique [0002] Beijing oil chicken is a local fine breed unique to the Beijing area, with excellent meat quality and eggs, and a unique appearance. It is one of the precious local breed resources among the 27 chicken breeds listed in the "China Poultry Breeds". Beijing oil chicken is basically in a state of small-scale conservation. The number of chickens in the country does not exceed 6,000, and it was once on the verge of extinction. In 2000, it was included in the "National List of Livestock and Poultry Breed Resources Protection" by the Ministry of Agriculture for the first time. [0003] The current methods for identifying poultry populations are mainly through chicken breed morphological markers and production performance measurement, which has certain limitations and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 文杰赵桂苹刘冉冉崔焕先郑麦青陈继兰王慧华李鹏
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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