Ming classification human expansive type gastric cancer cell line and application thereof

A technology of gastric cancer cell lines and gastric cancer cells, applied in the field of microbial animal cell lines, can solve the problems that there are not many and Ming's gastric cancer cell lines have not been reported, and achieve the effect of strong clone formation ability

Active Publication Date: 2013-11-13
ZHONGSHAN HOSPITAL XIAMEN UNIV
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  • Abstract
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Problems solved by technology

[0006] So far, there have been many reports on the clinical and basic research of gastric cancer in the world, but there are not many reports

Method used

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  • Ming classification human expansive type gastric cancer cell line and application thereof
  • Ming classification human expansive type gastric cancer cell line and application thereof
  • Ming classification human expansive type gastric cancer cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] The experimental methods described in the following examples are conventional methods unless otherwise specified.

[0029] Establishment and identification of Ming's expanded gastric cancer cell line XGC-2.

[0030] 1. Establishment of Ming's expanded gastric cancer cell line XGC-2

[0031] Tumor tissues from patients with gastric cancer were clinically collected, digested with mixed enzymes and cultured as primary cultures, and a gastric cancer cell line capable of continuous passage was successfully established. It has been passed down to 62 passages, and the cell properties remain stable. The patient's pathological findings were moderately differentiated tubular adenocarcinoma in the cardia of the gastric fundus ( figure 1 ).

[0032] 2. Detection of biological characteristics of Ming's expanded gastric cancer cell line XGC-2

[0033] 1. Cell morphology: After the cells grow stably and transfer, observe the cultured cells as live cells; in addition, the monolayer ...

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Abstract

The invention provides a Ming classification human expansive type gastric cancer cell line and application thereof, and relates to a microorganism animal cell line. The Ming classification human expansive type gastric cancer cell line is a Ming classification human expansive type gastric cancer cell strain XGC-2 with the preservation number of CCTCC (China Center for Type Culture Collection) NO:C201386. The Ming classification human expansive type gastric cancer cell strain XGC-2 can be applied in a cell model of occurrence, development or transfer of Ming classification expansive type gastric cancer, applied to establishment of a Ming classification expansive type gastric cancer animal model, applied to a cell model for researching the differentiation mechanism, cellular shape and function abnormality, tumor invasion and metastasis mechanism and clinical comprehensive diagnosis guidance and the like from the aspect of Ming classification types, and applied to research of gastric cancer occurrence mechanism and screening of gastric cancer preventing and treating medicaments.

Description

technical field [0001] The invention relates to microbial animal cell lines, in particular to a Ming's expanded gastric cancer cell line and its application. Background technique [0002] Gastric cancer is one of the most common malignant tumors. In the world, the incidence of gastric cancer ranks fourth among all malignant tumors, and the mortality rate ranks second. In China, gastric cancer is the most common digestive tract tumor, accounting for the first place in the mortality rate of malignant tumors. The national average annual mortality rate is 16 / 100,000 population, and the high-incidence area reaches 60 / 100,000 population. Although great progress has been made in the diagnosis and treatment of gastric cancer, its 5-year survival rate has not improved significantly in the past 10 years. Postoperative recurrence and metastasis are the important causes of its death. The research and treatment of gastric cancer has become a major issue to be solved urgently. Current d...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02A01K67/027
Inventor 蔡建春徐浩彭积贵骆启聪黄卫锋庄伊凡陈嘉佳
Owner ZHONGSHAN HOSPITAL XIAMEN UNIV
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