Anti-liver cancer stem cell monoclonal antibody and its application
A monoclonal antibody and stem cell technology, applied in the direction of antibodies, anti-tumor drugs, anti-animal/human immunoglobulin, etc., can solve the problems of clinical application impact, unsatisfactory specificity of anti-liver cancer monoclonal antibody, etc., and achieve The effect of strong proliferative ability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0020] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
[0021] 1. Mouse anti-human liver cancer stem cell PLC-Nanog POS Preparation of monoclonal antibodies
[0022] 1. Mouse anti-human liver cancer stem cell PLC-Nanog POS Obtaining hybridoma cell lines
[0023] The cultured PLC-Nanog POS Nanog neg Cells (i.e. liver cancer cells PLC with Nanog as promoter to regulate GFP expression) were sorted by flow cytometry to select PLC-Nanog POS Cells (i.e. Nanog-positive PLC cells) were used as immunogens; healthy BALB / c mice aged 4-6 weeks with a body weight of 14-18 g were injected intraperitoneally with 1×10 6 PLC-Nanog POS The cells were immunized for the first time, and then boosted immunized twice in the same way every 2 weeks. On the 10th day after the last booster immunization, the tail blood of the mice was collected, the serum was separated, and the antibody titer was detected by the ind...
PUM
![No PUM](https://static-eureka.patsnap.com/ssr/23.2.0/_nuxt/noPUMSmall.5c5f49c7.png)
Abstract
Description
Claims
Application Information
![application no application](https://static-eureka.patsnap.com/ssr/23.2.0/_nuxt/application.06fe782c.png)
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap