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Container and method for processing, sterilizing and preserving biological tissue

A technology of biological tissue and storage container, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of complex tissue and organ matrix process, and achieve the effect of reducing damage and damage

Active Publication Date: 2015-09-23
BEIJING RUIJIAN GAOKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The process of preparing tissue and organ matrices is very complicated

Method used

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  • Container and method for processing, sterilizing and preserving biological tissue
  • Container and method for processing, sterilizing and preserving biological tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]About 0.8 mm thick dehaired pig dermis was put into 0.1% polyethylene glycol octyl phenyl ether solution, and ultrasonic cleaning was used to remove blood and surface dirt for 1 hour. Rinse twice with normal saline, 60 minutes each time. Cut into rectangles 14 cm long and 10 cm wide, put in a rectangular multipurpose disposal container 20 cm long and 16 cm wide, and seal. Add 100 milliliters of calcium chloride containing 2.0 millimoles per liter and 100 units of dispase from the liquid inlet, and place on a shaker at 37 degrees Celsius for 20 hours to decellularize. Drain the dispase solution from the liquid outlet, add 100 ml of 1.0% sodium deoxycholate solution from the liquid inlet, decellularize and wash for 24 hours on a shaker at 37 degrees Celsius. Discharge the sodium deoxycholate solution from the liquid outlet, and add 100 ml of normal saline from the liquid inlet to rinse for 60 minutes. Drain the normal saline flushing solution from the liquid outlet, and ...

Embodiment 2

[0037] About 0.8 mm thick dehaired pig leather, soaked in 1% ammonia water for 16 hours. Rinse with pure water for 1 hour after soaking in ammonia water. Cut into rectangles 14 cm long and 10 cm wide, put them in a rectangular multipurpose container 20 cm long and 16 cm wide, and seal. Add 100 ml of 1.0% sodium deoxycholate solution from the liquid inlet to decellularize and wash for 24 hours on a shaker at 37 degrees Celsius. Drain the sodium deoxycholate solution from the liquid outlet, add 100 ml of normal saline from the liquid inlet to rinse for 60 minutes, and rinse repeatedly three times. Drain the normal saline flushing solution from the liquid outlet, and add 100 ml of 3% hydrogen peroxide from the liquid inlet to treat for 4 hours. Discharge hydrogen peroxide from the liquid outlet, add physiological saline from the liquid inlet to rinse for 60 minutes, and rinse repeatedly three times. Finally, the flushing solution was discharged, and 15 ml of normal saline solu...

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PUM

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Abstract

The present invention provides a multifunctional container for processing and storing biological tissue. The container comprises a container main body, for providing a processing space. The main body comprises an unsealed edge; at least one inlet, each inlet being provided with a filter; and at least one outlet, each inlet and each outlet being provided with a valve. The container main body is further provided with at least one peelable angle. The multifunctional processing and storage container can be used in production and can be further used as a container for protecting a terminal product. The multifunctional container can simplify a procedure of preparing a biological tissue matrix and improve the efficiency. A biological tissue material does not need an aseptic operation or get away from the container from the time when the biological tissue material enters the container to the time when the terminal product is used. The present invention further provides a method for processing and packing biological tissue.

Description

technical field [0001] The invention relates to a container which can be used for biological tissue processing, terminal sterilization and storage and its application method. Background technique [0002] The processing of biological tissues and organs and the processing of biological materials are often carried out in a sterile, dust-free, and relatively isolated environment to avoid contamination of tissue materials by contamination sources from the processing environment, cross-contamination between tissue materials, or tissue material reverse pollution of the environment. In actual operation, special facilities and unique technological processes are mainly used to avoid cross-contamination of tissue materials or environmental pollution. [0003] In the medical field, allogeneic or xenogeneic biological tissue and organ materials need a series of biological treatments before they are used in human tissue repair and repair in clinical operations, including cleaning, disin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0263A01N1/0215
Inventor 刘志刚刘新华
Owner BEIJING RUIJIAN GAOKE BIOTECH