Rejuvenation culture method for artificial breeding of cordyceps militaris strain

A Cordyceps strain and artificial breeding technology, applied in the fields of botanical equipment and methods, cultivation, plant cultivation, etc., can solve the problems of artificial breeding Cordyceps fungus species degradation and other problems, so as to solve the problem of species degradation and solve sustainable production. Effect

Active Publication Date: 2015-04-15
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of species degeneration of artificially propagated Cordyceps strains, the invention provides a method for rejuvenating species of artificially propagated Cordyceps strains, comprising the following steps:

Method used

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  • Rejuvenation culture method for artificial breeding of cordyceps militaris strain
  • Rejuvenation culture method for artificial breeding of cordyceps militaris strain
  • Rejuvenation culture method for artificial breeding of cordyceps militaris strain

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Embodiment 1

[0020] Example 1 The screening and investigation of bacteria-infected host larvae

[0021] Collect 1500 larvae from each of the six groups of bat moth artificially reared for 1-2 months, and name them as experimental group Ⅰ, experimental group Ⅱ, experimental group Ⅲ, experimental group Ⅳ and experimental group Ⅴ. Collect and cultivate Cordyceps sinensis through the method described in the patent application "A low-altitude artificial cultivation method of Cordyceps sinensis" (application number: CN201210292550.6) submitted by the applicant, and the ascospores, conidia and bacterial skin of Cordyceps sinensis cultivated from this method are used as bacteria. According to this method, the Cordyceps sinensis is cultivated repeatedly; the above operations are performed more than 3 times, and the ascospores, conidia and bacterial skins obtained by harvesting are used as propagation materials, and these propagation materials are mixed as artificial multiplication propagation materi...

Embodiment 2

[0028] The investigation of the activity of embodiment 2 rejuvenation strain propagating material

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Abstract

Disclosed is a method for natural species rejuvenation of artificially bred strains, comprising the following steps: 1) infecting cordyceps host larvae with cordyceps strains artificially bred for more than 2 generations, screening the contaminated larvae which are firstly rejuvenated, and placing the larvae in a rejuvenation base after continuously culturing for 1 to 2 stages; 2) managing the transition periods so that the contaminated larvae adapt to the wild environment; 3) managing the specie nature exercise period of the bombyx insects; 4) rejuvenation of natural species of sexual spores and mycodermas; 5) collecting cordyceps with mature sexual spores, and transplanting to a sterilized culture medium to continuously culture after washing the insect-strain complexes; 6) after-ripening of the cordyceps; and 7) harvesting cordyceps strain materials. The method provides a strain guarantee for large-scale sustainable production of cordyceps.

Description

technical field [0001] The invention relates to a strain selection and breeding technology, in particular to a rejuvenation culture method for artificially breeding Cordyceps strains. technical background [0002] Cordyceps mainly refers to Cordyceps sinensis, and it is also a combination of fungi and insects formed by other fungi of the family Ergotaceae (Cordyceps fungus) and larvae of the family Batmothidae under special conditions, including Cordyceps sinensis, Liangshan Cordyceps and Cordyceps militaris, etc.; Cordyceps sinensis, is Rare and valuable Chinese herbal medicines unique to our country. It has the functions of invigorating the lungs and kidneys, relieving cough and reducing phlegm, and mainly treating diseases such as cough, hemoptysis, impotence and nocturnal emission, physical weakness after illness, and chronic cough in the elderly. Cordyceps sinensis is mainly produced in Tibet, Qinghai, Yunnan, Sichuan, Gansu and other provinces in my country, in local ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
CPCA01G1/04A01G18/00
Inventor 刘飞
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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