A kind of method for rapid propagation of Radix chinensis tissue culture

A technology of tissue culture and lotus, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of reduced yield, consumption of large tubers, low germination rate of seeds, etc., to improve yield and quality, and promote industrial development The effect of chemical production and ensuring the purity of seedlings

Inactive Publication Date: 2015-12-23
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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Problems solved by technology

[0003] Although the lotus blooms and bears fruit, the germination rate of the seeds is low and the growth is slow
However, the use of underground stems grows fast, and a small amount of harvest can be harvested in the year of planting. Therefore, the conventional propagation method in production is tuber division propagation. However, this asexual reproduction method will cause the accumulation of viruses as the number of planting generations increases. Deterioration and yield reduction, in addition, when carrying out large-scale planting, need to consume a large amount of seed tubers, increased planting cost, and tissue culture rapid propagation technology has become the effective way to solve these problems, therefore, the tissue culture rapid propagation of the lotus The application of traditional technology can provide technical support for its mass production

Method used

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Embodiment Construction

[0016] A kind of method for the fast propagation of the lotus tissue culture, concrete steps are:

[0017] Take the tip of the young tuber of the lotus root as the explant, after disinfection treatment, strip off the growth point of 0.2 mm in size under the vertical microscope, transfer it to the adventitious bud induction medium for cultivation, and transfer it when the adventitious bud grows to 1.5 cm. Into the strong seedling medium, the culture conditions of test tube seedlings are: temperature 25°C, light 1500Lx / 12h.d, pH 6.0;

[0018] When the test-tube seedling grows to 3cm, transfer it to the rooting medium. When the root length is 5cm, harden the strong rooting seedling, remove the rooting bottle seedling to the culture rack, place it at room temperature and under natural light to adapt to the natural environment, and exercise for 5 days , and then open the bottle cap to clean the root agar, transplant it into the vermiculite substrate, pour the fixed root water thoro...

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Abstract

The invention discloses a method for rapid breeding in zingiber mioga tissue culture. The method mainly comprises the following steps: with a tender tuber tip as an explant, after sterilization treatment, peeling a growth point of 0.1-0.3mm under a vertical microscope, inoculating into an adventitious bud induction medium, further transferring the adventitious bud into strong seedling culture, subsequently transferring healthy and strong test tube seedlings into a rooting medium, and finally hardening and transplanting the test tube seedlings. Due to adoption of the method, not only are effects of culture and detoxification rapid breeding of zingiber mioga tissues achieved, but also the breeding efficiency is improved, the production period is shortened, and an effective way is provided for in-scale breeding of zingiber mioga.

Description

technical field [0001] The invention relates to the field of tissue culture rapid propagation of medicinal plants, in particular to a method for tissue culture rapid propagation of lotus. Background technique [0002] 蘘草 ( Zingiber Mioga Rosc ) is also known as Yanghuo, 蘘草, wild ginger, Minghe, etc. It is a perennial herb of the Zingiberaceae genus, and the main edible part is the flower axis. It has medicinal effects such as promoting blood circulation and regulating menstruation, antitussive and expectorant, detumescence, detoxification, eliminating stagnation and invigorating the stomach. The lotus root is rich in various vitamins, iron, zinc, selenium and other trace elements, cellulose, aromatic esters, etc., and has a delicious fragrance , is a dietary fiber vegetable with high nutritional value and homologous food and medicine. It mainly feeds on the buds and flower spikes of the lotus, including the fleshy scales outside the flower spikes. Xianghe is rich in dieta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 江芹朱培蕾廖华俊刘才宇董玲赵贵云宁志怨蒋君汤婷婷李卫文
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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