SERS (Surface Enhanced Raman Spectroscopy) method for detecting alpha fetal protein based on probe-marked immunogold and magnetic composite materials

A magnetic composite material and alpha-fetoprotein technology, applied in the field of protein analysis and detection, can solve problems such as radioactive pollution, narrow linear range, and poor signal-to-noise ratio

Inactive Publication Date: 2013-11-27
JILIN UNIV
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Problems solved by technology

For example, the time-resolved fluorescent immunoassay method has poor signal-to-noise ratio and low detection line; the enzyme-linked immunosorb

Method used

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  • SERS (Surface Enhanced Raman Spectroscopy) method for detecting alpha fetal protein based on probe-marked immunogold and magnetic composite materials
  • SERS (Surface Enhanced Raman Spectroscopy) method for detecting alpha fetal protein based on probe-marked immunogold and magnetic composite materials
  • SERS (Surface Enhanced Raman Spectroscopy) method for detecting alpha fetal protein based on probe-marked immunogold and magnetic composite materials

Examples

Experimental program
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Embodiment 1

[0045] Example 1: SERS immunodetection of alpha-fetoprotein

[0046] It mainly includes the following steps:

[0047] (a) Prepare a gold nanoparticle solution modified with P-mercaptobenzoic acid (MBA) molecules, then combine with the alpha-fetoprotein antibody, and then block with BSA reagent;

[0048] (b) Preparation of SiO 2 Coated Fe 3 o 4 Magnetic composite materials;

[0049] (c) Preparation of aldylated SiO 2 Coated Fe 3 o 4 Magnetic composite material, and formulated into PBS dispersion;

[0050] (d) AFP antigen solutions with different concentrations were prepared in PBS and mixed with the aldylated SiO 2 Coated Fe 3 o 4 The magnetic composite materials are mixed in equal volumes, and then sealed with an equal volume of BSA reagent;

[0051] (e) Alpha-fetoprotein antigen and SiO on gold nanoparticles 2 Coated Fe 3 o 4 The alpha-fetoprotein antibody on the magnetic composite material undergoes an immune reaction and is incubated at 37°C for 1.5 hours;

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Abstract

The invention relates to an SERS (Surface Enhanced Raman Spectroscopy) method for detecting alpha fetal protein based on a probe-marked immunogold and magnetic composite material, and belongs to the technical field of protein analysis detection. The method comprises the following steps: combining P-thiosalicylic acid (MBA)-molecule-modified gold nanoparticle particles with an alpha fetal protein antibody, preparing a surface-functionalized Fe3O4@SiO2 magnetic composite material, absorbing the alpha fetal protein in serum onto the functionalized Fe3O4@SiO2 magnetic composite material; performing an immune reaction on an immunogold probe and the Fe3O4@SiO2 magnetic composite material modified with an alpha fetal protein antigen; performing Raman spectrum detection. The concentration of the alpha fetal protein can be detected by substituting the Raman strength value of an obtained Raman spectrum characteristic peak of protein to be detected into a working curve of Raman strength value and alpha fetal protein antigen concentration. The method has the advantages of ultrasensitiveness, high selectivity, mild detection conditions and the like, and has extremely important meanings in clinical analysis and diagnosis.

Description

technical field [0001] The invention belongs to the technical field of protein analysis and detection, in particular to a probe-based immunogold-Fe 3 o 4 A surface-enhanced Raman spectroscopy (SERS) method for the detection of alpha-fetoprotein levels in serum using magnetic composite materials. Background technique [0002] Cancer is still one of the incurable diseases so far. Due to factors such as population growth and increasing life expectancy, the number of cancer patients is increasing year by year around the world. According to the current conventional therapy recognized by the international medical community, the treatment of cancer generally requires surgical resection combined with radiotherapy and chemotherapy. However, radiotherapy and chemotherapy often kill cancer cells and seriously damage the functions of multiple organs in the human body. For this reason, scientists have been working hard to find better and more direct ways to treat cancer with fewer sid...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N33/68G01N33/531
Inventor 王旭王爱敬赵冰宋薇阮伟东陈雷
Owner JILIN UNIV
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