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Kit

A kit and the technology of schizoside A, applied in the field of medical devices, can solve the problem of low content and achieve the effect of strong sensitivity and stability

Active Publication Date: 2013-12-11
SHUNHAO CELL BIOTECHNOLOGY (TIANJIN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the decades after the discovery of this factor, the greatest difficulty of research was that its content in the body was very low, and because the structure has not yet been determined, there is currently no way to isolate it (them) from the body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: The kit is used to measure the content of endogenous digitalis-like factor secreted by placental tissue

[0055] (1) Collect the placenta, cut into 2cm×2cm×2cm tissue pieces, freeze in liquid nitrogen, and store at -80°C;

[0056] (2) Cut the frozen placental tissue into slices, place in a homogenizer, and freeze in a liquid nitrogen tank for 1 minute; homogenize the tissue slices at a speed of 1500 rpm for 5 minutes;

[0057] (3) Collect the homogenized tissue, add an equal amount of methanol, mix well, and centrifuge at 2000rpm for 5 minutes to remove protein; collect the centrifuged supernatant, concentrate in vacuo, and store at 4°C;

[0058] (4) Take out the digoxin antibody, secondary antibody, and radioactively labeled sarcoside A from the kit, add them in equal amounts, mix well, and incubate at room temperature;

[0059] (5) Then add an equal amount of PEG6000 solution and centrifuge to obtain a precipitate;

[0060] (6) The precipitate was resuspe...

Embodiment 2

[0072] Example 2 This kit is used to measure the content of endogenous digitalis-like factor in human frozen placenta tissue

[0073] (1) Cut the frozen placental tissue into thin slices with a blade;

[0074] (2) Place the tissue slices in a homogenizer and freeze in liquid nitrogen for 1 minute;

[0075] (3) Homogenize at a speed of 1500rpm for 5 minutes;

[0076] (4) Take out the tissue homogenate, add an equal amount of methanol, and centrifuge to remove protein;

[0077] (5) Concentrate and store the supernatant;

[0078] The method for measuring samples is the same as steps (4)-(7) in Example 1. Standard curve is drawn with embodiment 1.

[0079] Such as Figure 4 As shown, this kit can measure the concentration of endogenous digitalis-like factors in frozen placenta tissue, and the samples are all from people who have been diagnosed with preeclampsia.

Embodiment 3

[0080] Embodiment 3: The kit is used to measure the content of endogenous digitalis-like factor in human serum

[0081] The measurement method is the same as steps (4)-(7) in Example 1. The result is as Figure 5 As shown, the kit can accurately measure low levels of endogenous digitalis-like factor in human serum.

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Abstract

The invention belongs to the technical field of medical appliances, and discloses a kit which comprises primary antibody digoxin antibody, secondary antibody rat anti-goat IgG, 3H labeled divaricoside, polyethyleneglycol 6000, phosphate buffer solution (PBS) with the pH value of 7.4, 3H scintillation solution, and tris(hydroxymethyl)metyl aminomethan buffer solution (Tris buffer solution) with the pH value of 7.4, and / or divaricoside. The kit provided by the invention is simple and convenient to operate, has the advantages of high sensitivity, high specificity and the like, and can perform stable and reliable early diagnosis on gestational period eclamptism, thereby providing favorable support for subsequent treatment.

Description

technical field [0001] The invention belongs to the technical field of medical devices, in particular to a kit for detecting preeclampsia or eclampsia during pregnancy. Background technique [0002] Preeclampsia during pregnancy is a syndrome with complex etiology. If left untreated, it can develop into eclampsia, which is life-threatening. The main symptoms are high blood pressure, proteinuria, edema, convulsions, renal function damage, etc. It has become a disease with the highest mortality rate among pregnant women in the world, and the specific etiology is currently unknown. Studies have shown that a substance produced by the placenta that causes maternal vascular endothelial dysfunction in susceptible women may be one of the reasons for the onset of preeclampsia. Elevated blood pressure is the most obvious hallmark of the disease, causing damage to the maternal endothelium, kidneys and liver, and releasing vasoconstrictors. [0003] Preeclampsia affects 6-8% of pregna...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/541
Inventor 李相国
Owner SHUNHAO CELL BIOTECHNOLOGY (TIANJIN) CO LTD
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