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Method for producing hirsutella sinensis with solids

A Chinese Trichospora, solid technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult mass production, many operation steps, and low effective components for kidney health care. Significant kidney-enhancing biological activity, reduced levels, significant kidney health effects

Active Publication Date: 2014-01-01
QINGHAI JIUSHI WORM GRASS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both of the above two patents use the fermentation method of Mortierella species in China to produce bacterial powder. The disadvantage is that there are many operating steps, it is not easy to produce on a large scale, and the active ingredients in the bacterial powder have low kidney health effects.

Method used

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  • Method for producing hirsutella sinensis with solids
  • Method for producing hirsutella sinensis with solids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step 1: Crush 90g of crushed dry wolfberry, 5g of millet, and 5g of mung beans respectively, pass through a 10-mesh sieve, mix with 100g of milk, put them into a culture container, the culture medium accounts for 7 / 10 of the volume of the culture container, and sterilize with damp heat at 125°C Bacteria for 30 minutes, add 0.5g Mortierella sinensis species; culture at 22°C for 3 days, the surface of the medium can be covered with white hyphae;

[0030] Step 2: Take out the culture and place it in a drying oven, dry it, crush it, and pass it through a 120-mesh sieve to obtain 40 g of Mortierella sinensis powder.

Embodiment 2

[0032] Step 1: Crush 20g of wolfberry juice, 40g of wheat, and 40g of peas respectively, pass through a 10-mesh sieve, mix with 100g of goat milk, put them into a culture container, the culture medium accounts for 1 / 10 of the volume of the culture container, and sterilize with damp heat at 140°C For 30 minutes, insert 1g Mortierella sinensis species; culture at 15°C for 9 days, the surface of the culture medium is covered with hyphae;

[0033] Step 2: Take out the culture and put it in a drying oven, dry it, crush it, and pass it through a 120-mesh sieve to obtain 43g of Mortierella sinensis powder.

Embodiment 3

[0035] Step 1: Crush 59g of fresh wolfberry fruit, 1g of rice, and 40g of peas, pass through a 10-mesh sieve, mix with 100g of horse milk, put them into a culture container, the culture medium accounts for 3 / 10 of the volume of the culture container, and sterilize with damp heat at 130°C For 30 minutes, add 0.8g Mortierella sinensis species; culture at 18°C ​​for 8 days, and the surface of the medium will be covered with white hyphae;

[0036] Step 2: Take out the culture and put it in a drying oven, dry it, crush it, and pass it through a 120-mesh sieve to obtain 35g of Mortierella sinensis powder.

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Abstract

The invention discloses a method for producing hirsutella sinensis with solids, and relates to a production method, which is used for producing the hirsutella sinensis by a solid culture medium, of the hirsutella sinensis. The method is characterized by comprising steps as follows: step one, 20%-90% of wolfberries, 1%-40% of grains and 5%-40% of beans are mixed, the same mass of milk is added, the mixture is placed into a culture container after prepared according to the weight percentage, the culture medium occupies 1 / 10-7 / 10 of the volume of the culture container, moist heat sterilization is performed for 30 minutes at the temperatures ranging from 115 DEG C to 140 DEG C, hirsutella sinensis strains which are 0.5%-1% of the weight of the culture medium are inoculated and cultured at the temperature of 15 DEG C-22 DEG C for 3-9 days, and mycelia grows all over the surface of the culture medium; and step two, a culture is dried, crushed and sieved to obtain hirsutella sinensis powder. According to the method, large-scale industrial production is facilitated, the hirsutella sinensis can be produced with the solids fast, efficiently, environmentally and safely, and the obtained hirsutella sinensis powder has obvious kidney health care effects.

Description

Background technique [0001] Cordyceps sinensis is a complex of insects and bacteria formed after the larvae of Hepialus armoricamus Oberthur of the genus Lepidoptera are infected with the fungus Mortierella chinensis. Chinese patent CN101530042 discloses the Chinese Mortierella powder fermentation production process, and CN101423799 discloses a Chinese Mortierella production method. Both of the above two patents use the fermentation method of Mortierella species to produce bacterial powder. The disadvantages are that the operation steps are numerous, it is not easy to produce on a large scale, and the active ingredients in the bacterial powder have low kidney health effects. Contents of the invention [0002] The purpose of the present invention is to overcome the deficiencies of the prior art, to provide the use of natural wolfberry as one of the culture medium raw materials, which is easy for large-scale industrial production, and can be used for fast, efficient, green and...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 涂峰李桂全张玉业
Owner QINGHAI JIUSHI WORM GRASS BIOTECH
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