Pathology application of schizocapsa total saponins

A technology of total saponins, split fruit potato, applied in the field of medical pathology

Inactive Publication Date: 2015-05-06
周莉 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the treatment of liver injury secondary to diabetes mellitus

Method used

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  • Pathology application of schizocapsa total saponins
  • Pathology application of schizocapsa total saponins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Determination of the inhibitory constant of CYP2E1 enzyme activity by total saponin

[0025] CYP2E1 catalyzes the aniline hydroxylation reaction, so aniline hydroxylase is a probe for CYP2E1, that is, the determination of aniline hydroxylase activity can indicate the level of CYP2E1 activity. Take normal animal livers and determine the activity of aniline hydroxylase. The activity of aniline hydroxylase increases with the increase of substrate concentration, showing saturation kinetic characteristics, which conforms to the Michaelis-Menten equation. Calculated K m The 95% confidence interval of the value is 22.18 to 43.24μmol / L, V max The value is 0.48 μmol / min / mg.

[0026] According to the experimental results, the concentration of aniline was selected as 50μmol / L, and the IC of total saponins of yam 50 Value (half inhibitory concentration) determination experiment. The total saponins of schistophylla are dissolved in water to form a concentration gradient and added to th...

Embodiment 2

[0028] Experimental Study on the Protective Effect of Total Saponins of Schizophyta on Liver Damage Secondary to Diabetes

[0029] Compared with the control group, the activities of serum glutamate aminotransferase (sALT), aspartate aminotransferase (sAST) and CYP2E1 (aniline hydroxylase) were significantly increased in the diabetic group (all P<0.05). Indicates secondary damage to the liver.

[0030] Compared with the diabetic group, the liver CYP2E1 activity, serum glutamate aminotransferase (sALT) and aspartate aminotransferase (sAST) in the total saponins group of Schistocarpus saponins were significantly reduced (all P<0.05), indicating that the total saponins of Schistocarpa It can protect secondary liver damage due to diabetes.

[0031] Table 2 The effect of total saponins of schistophylla on secondary liver injury in diabetes. n (number of cases) = 15, x ± s (mean ± standard deviation)

[0032]

[0033]

[0034] Note: *P<0.01; **P<0.05

Embodiment 3

[0036] In Vitro Experimental Study of Total Saponins of Schizophyta on Inhibiting High CYP2E1 Activity Secondary to Diabetes

[0037] In diabetic rats, liver microsomal CYP2E1 activity, that is, aniline hydroxylase activity, was 0.82±0.05μmol / min / g, which was 2.3 times higher than that of the control group with aniline hydroxylase activity 0.35±0.12μmol / min / g. In the in vitro incubation system, adding different concentrations of total saponins of schistophylla (take the raw material of saponins of schizophyta, dilute to different concentrations, and add to the in vitro incubation system), the activity of CYP2E1 in the diabetic group was significantly inhibited and was concentration-dependent.

[0038] The above experimental results show that the total saponins of yam has the effect of treating the liver function damage secondary to diabetes and can be used for clinical treatment.

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Abstract

The invention relates to pathology application of schizocapsa total saponins in preparing medicine for treating liver damage secondary to diabetes. The schizocapsa total saponins is capable of inhibiting CYP2E1specifically so as to treat liver function damages secondary to the diabetes, and is a hepatoprotective medicine with high medical and economic value.

Description

Technical field [0001] The invention relates to medical pathology, in particular to the use of the total saponins of the yam yam in the preparation of a medicine for treating the liver function damage secondary to diabetes. Background technique [0002] The liver is the body's main organ for the transformation and metabolism of various substances. It expresses a variety of enzyme systems involved in substance metabolism, the most important of which is cytochrome P450 (CYPs). In CYPs, subtype 2E1 (CYP2E1) mainly metabolizes small molecule compounds, including a variety of drugs, halogenated hydrocarbons, alcohols, ketones, and endogenous substances such as arachidonic acid and fatty acids. CYP2E1 is considered to be an important determinant that affects the body's sensitivity to various toxins and carcinogenicity, especially in mediating liver damage. It is known that in the liver lobules, the expression level of CYP2E1 in hepatocytes located around the central vein is 30 times h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/88A61P3/10A61P1/16
Inventor 周莉王敏
Owner 周莉
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