Method for utilizing lilium brownie roots to induce callus and embryoid

A callus and embryoid technology is applied in the field of using lily root to induce callus and embryoid, which can solve the problem of no useful roots and the like, and achieve the effects of reducing pollution opportunities, saving energy, and simple induction medium.

Inactive Publication Date: 2014-02-12
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for quicker and more efficient production of plant cells from sterility female parental shoots (SOPs). It involves growing these seeds into tissue pieces called cotyledons or tubers by inducing different stages during their development cycle. These induced cellular structures produce embryo body(EB), which then develop further through various steps like germination, stem elongating, dormancy, sprouting, etc., resulting in fast reproductions over several months' time with minimal environmental impact compared to traditional methods such as incubations.

Problems solved by technology

In this patented technical problem addressed in this patents relates to improving the efficiency at producing high yields of lile plants due to their ability to reproduce without being affected during different stages of vegetative multiplication. Existing techniques like seeding involve developing shoots into tubers before growing them outwardly, while tissue cultivating involves inducing stem cells called cormules to develop young foliage. These conventional approaches require many steps and may result in reduced productivity rates compared to natural processes.

Method used

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  • Method for utilizing lilium brownie roots to induce callus and embryoid
  • Method for utilizing lilium brownie roots to induce callus and embryoid
  • Method for utilizing lilium brownie roots to induce callus and embryoid

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Embodiment 1

[0029] (1) Preparation of culture medium:

[0030] Induction medium: MS medium 1L, sucrose 30000mg / L, agar powder 5000mg / L, and special ingredients composed of 6-BA0.5mg / L, PIC1.0mg / L and hydrolyzed casein 500mg / L.

[0031] Growth and rooting medium: MS medium 1L, BA 0.2mg / L, HMI 0.2mg / L.

[0032] After preparation, adjust pH=5.4-5.6, 121±2℃, sterilize 15-20 pounds for 20 minutes, set aside.

[0033] (2) Select the roots of the aseptic lily seedlings, inoculate them in the induction medium for dark culture, at a temperature of 25±2℃, and produce callus in about 30 days (see figure 1 ), gradually produce embryoid bodies (see figure 2 ).

[0034] (3) The embryoid body is peeled off about 50 days, transferred to the growth and rooting medium, cultured in the light, the temperature is 25±2℃, the light is 16 hours a day, and it gradually turns green (see image 3 ).

[0035] (4) Normal plants that grow into roots after 1 month of cultivation (see Figure 4 ).

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Abstract

A disclosed method for utilizing lilium brownie roots to induce callus and embryoid comprises the following steps: 1) roots of lilium brownie aseptic seedling are selected and inoculated to an induction medium for dark culture with the culture room temperature of 23-27 DEG C; 2) embryoids on callus obtained by dark culture are transplanted to a growth rooting medium for culture in light with illumination for 16-20 h every day at a temperature of 23-27 DEG C; and 3) the embryoids are greened, gradually grow into normal plants, and root. By employing the specific culture method and the culture medium provided by the invention, the inductivity of callus induced by lilium brownie roots reaches 100%, all root explants are overgrown with calluses after cultured for about 50 days, the embryoid inductivity is 90% and the plants grow well.

Description

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Claims

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Application Information

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Owner SHANGHAI ACAD OF AGRI SCI
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