The invention discloses a taxus chinensis cuttage cultivation method. The taxus chinensis cuttage cultivation method comprises the steps as follows: inserting insertion branches treated with plant hormones onto a prepared seedbed substrate in sequence, controlling the cuttage depth to be 3-6 cm, watering each day, spraying a 700-time carbendazol solution once each week, after half a month, reducing the watering frequency, averagely watering once every 3 days, spraying the 700-time carbendazol solution once every two weeks, and transplanting for cultivating after the insertion branches take roots. The method has the advantages that firstly, as the seedbed substrate adopted in the method is better in physical permeability, formation, rooting and after-rooting growth of callus tissues are facilitated; secondly, saw powder is good in heat retaining property and the temperature difference between day and night for the substrate is slightly changed, so that rooting is facilitated; and thirdly, a small amount of humus soil is strong in water and fertility retaining capability, so that growth of root systems and new branches of nursery stocks is facilitated; the seedbed substrate is disinfected at the same time, so that the insertion branches are effectively prevented being infected with pathogenic bacteria; and the insertion branches are sprayed with the carbendazol solution after cuttage, so that the cuttage rooting is facilitated, the survival rate is increased, and the seedling growth time is shortened.