30 results about "Callisia repens" patented technology

The invention discloses a roof greening method by using callisia repens, in particular to a growing method for planting callisia repens on roof. The procedures of the method are as follows: first, a water-resistant system, a drainage system and a root resistant layer are arranged on the roof and a matrix layer is paved on the roof; second, grass seeds of the callisia repens is planted on the matrix layer by using any one method of offshoots, broadcasting sowing method, cottage or greensward transplanting and paving method; third, root fixing water is watered and N fertilizer is fertilized once every fifteen days, and lawn is formed within one to two months. As an effective measure for solving the influence of urban development on the entironment and raising the urban afforestation rate, the invention uses the callisia repens on the roof greening, which has a resultful effect on the heat resistance, virescence and disaster prevention of room surface.

The invention relates to a method for cultivating gardenia jasminoides, which belongs to the field of plant tissue culture, in particular to a method for cultivating the gardenia jasminoides and a non-pollution cultivation method thereof. The method comprises the following steps: disinfecting and cleaning the outer planting body of the gardenia jasminoides; inducing and differentiating the callus; proliferating the adventitious buds; inducing the adventitious roots; acclimatizing; and executing the non-pollution cultivation to realize the experiment purpose of the invention. The invention has the following beneficial effects: the invention obtains a large number of tissue culture seedlings to meet the planting needs, and overcomes the pollutions of pesticide and heavy metal in natural wood area. The survival rate is 95.5%, the weight of single plant is twice as the wild and asexual reproduction drug materials in the same year, and the effective components is higher than the wild drug materials in the same year. The invention meets the needs of original ingredients of pharmaceutical industries, not only cultivates in batches, but also plants the tissue culture seedlings in all areas, obtains the non-pollution drug materials two years in advance, and has practical value.

The invention discloses a taxus chinensis cuttage cultivation method. The taxus chinensis cuttage cultivation method comprises the steps as follows: inserting insertion branches treated with plant hormones onto a prepared seedbed substrate in sequence, controlling the cuttage depth to be 3-6 cm, watering each day, spraying a 700-time carbendazol solution once each week, after half a month, reducing the watering frequency, averagely watering once every 3 days, spraying the 700-time carbendazol solution once every two weeks, and transplanting for cultivating after the insertion branches take roots. The method has the advantages that firstly, as the seedbed substrate adopted in the method is better in physical permeability, formation, rooting and after-rooting growth of callus tissues are facilitated; secondly, saw powder is good in heat retaining property and the temperature difference between day and night for the substrate is slightly changed, so that rooting is facilitated; and thirdly, a small amount of humus soil is strong in water and fertility retaining capability, so that growth of root systems and new branches of nursery stocks is facilitated; the seedbed substrate is disinfected at the same time, so that the insertion branches are effectively prevented being infected with pathogenic bacteria; and the insertion branches are sprayed with the carbendazol solution after cuttage, so that the cuttage rooting is facilitated, the survival rate is increased, and the seedling growth time is shortened.

The invention discloses a culture method for ramulus uncariae cum uncis. The culture method comprises the following steps: firstly, culturing a ramulus uncariae cum uncis seed in a seed culture mediumfor 20-25d to obtain a sterile seedling hypocotyl; secondly, cutting a hypocotyl explant having the length of 0.4-0.8cm and containing no cotyledonary nodes from the sterile seedling hypocotyl, horizontally placing the hypocotyl explant in a first callus culture medium for dark culture for 20-25d, and then, transferring the hypocotyl explant into a second callus culture medium for dark culture toobtain a fresh callus; thirdly, transferring the fresh callus into a differential culture medium for differential culture to obtain a cluster bud; and fourthly, transplanting the cluster bud into a strong seedling culture medium for strong seedling culture, cutting off a basal part of a stem when the length of a single seedling is 3-4cm, transferring the seedling into a rooting culture medium forrooting culture, and growing a root with the length of 1.5-2.0cm to obtain a ramulus uncariae cum uncis seedling. The ramulus uncariae cum uncis obtained by using the culture method is purely natural, high in yield and high in medicinal component content.

A populus trichocarpa Torr.Gray leaf adventitious bud induction and plant regeneration method aims to solve the problems that at present, populus trichocarpa Torr.Gray has difficulty in tissue culture organogenesis and low in plant regeneration rate, and the adventitious bud inductivity is low and the period is long since populus trichocarpa Torr.Gray stems are taken as explants. The method provided by the invention comprises the following steps: 1, material collection and treatment; 2, inoculated culture and aseptic seedling expanding propagation: stems which are flushed by tap water are subject to sterile treatment for inoculated culture to propagate aseptic seedlings; 3, adventitious bud differentiation induction; 4, adventitious bud elongation induction: calluses with adventitious buds are cut into blocks and placed in an adventitious bud elongation culture medium for culture; 5, adventitious bud rootage culture; 6, hardening-seedling and transplanting. According to the invention, populus trichocarpa Torr.Gray aseptic seedling leaves are taken as explants, so that the adventitious bud inductivity and the survival rate are up to 100%, and from explant culture to seedling survival, only about 60 days are required. The populus trichocarpa Torr.Gray leaf adventitious bud induction and plant regeneration method is used for plant tissue culture.

The invention relates to a culture medium formula of plant callus induction and subculture multiplication of astragalus membranaceus (fisch.) bunge and a culture method of the astragalus membranaceus (fisch.) bunge. Four steps of sterilizing selected explants, sterilizing a culture medium, inducing callus induction and carrying out callus subculture multiplication are included. A plant callus culture method of astragalus membranaceus (fisch.) bunge comprises the following steps: by taking astragalus membranaceus (fisch.) bunge leaves as the explants, adding a culture medium prepared from maltose, glucose, sucrose, agar powder, sodium alginate, naphthylacetic acid and 6-benzyl purine and the like into an MS basic culture medium to carry out tissue culture to obtain astragalus membranaceus (fisch.) bunge leaf callus; adjusting the pH value of the culture medium, and filling triangular culture bottles with a prepared callus induction culture medium and a prepared callus subculture multiplication culture medium respectively, and covering ventilated plastic sealing films on bottle openings; and sterilizing at the pressure of 0.10-0.15MPa and carrying out callus culture. According to the culture medium formula and the culture method, provided by the invention, the astragalus membranaceus (fisch.) bunge leaf calluses can be effectively induced and rapidly multiplied, and a lot of the astragalus membranaceus (fisch.) bunge leaf calluses can be obtained in relatively short time; and the astragalus membranaceus (fisch.) bunge leaf calluses have the characteristics of short production period and rapid multiplication speed and are suitable for industrial production.

The invention discloses an establishing method of a Gleditsia vestita Chun et Howex B.G.Li.tissue culture regeneration system. The rapid propagation system of Gleditsia vestita Chun et Howex B.G.Li.tissue culture is established by taking cotyledons of aseptic seedlings of Gleditsia vestita Chun et Howex B.G.Li. as explants through callus inducement, adventitious bud proliferation, rooting and acclimatizating and transplanting. The results show that: the cotyledons of the Gleditsia vestita Chun et Howex B.G.Li.simultaneously induce callus and buds on a MS+6-BA3.0mg / L+KT0.4mg / L+IAA0.3mg / L culture medium, and the average induction rate reaches 90.5%; the best culture medium of adventitious bud proliferation is 1 / 2MS+6-BA1.0mg / L +IBA0.3mg / L+NAA0.2mg / L, and the proliferation multiple is 4.0; under the condition of MS+NAA0.5mg / L+IAA0.25mg / L, the rooting rate is up to 100%; plants after transplanting are good in growth vigor, and the survival rate is over 90%.

The invention provides a seedling cultivation method for kiwi fruit. The seedling cultivation method comprises the following steps of: grafting which is performed from the end of December to next January; sand storage, namely, seedlings subjected to the grafting are stored by river sand indoors; and planting of the seedlings in a nursery garden from February to the beginning of March. According to the invention, the grafting is performed in winter to avoid busy farming seasons of spring and summer, and the grafting time can be reasonably scheduled at daytime or at night as the grafting is performed indoors. After the seedlings subjected to the grafting are stored by the river sand indoors, most of the seedlings form callus at graft unions when being transplanted since an indoor temperature is high, thereby being beneficial to improving the rate of survival of the grafted seedlings. By virtue of the seedling cultivation method, the rate of survival of the grafted seedlings can reach 99%; and the pruning of stocks in a bleeding period is avoided, thus the nutrient loss of the stocks is reduced.

The invention discloses a method for inducing calluses of peony anther. A murashige and skoog (MS) culture medium is adopted to induce the calluses; and substances such as 6-benzylamino adenine (BA) cytokinin, a naphthylacetic acid (NAA) plant growth regulator, a 2,4-D herbicide and the like are prepared in the MS culture medium. The definite culture medium scheme and hormone combination are provided for a peony anther explant. The optimal material taking period of the calluses is presented. The inductivity of peony calluses is over 80 percent, and the better induction effect is achieved; and the inductivity of the calluses of the anther is over 50 percent.

The invention discloses a panacis majoris rhizoma tissue culture and in-vitro regeneration method. The panacis majoris rhizoma tissue culture and in-vitro regeneration method comprises selecting and disinfecting an explant, inducing callus, differentiating and inducing a bud, inducing rooting and the like. The tissue culture and the clone establishment of the panacis majoris rhizoma are explored to determine the ideal culture conditions for acquiring a sterile material, inducing the callus, differentiating the callus, forming the bud, rooting and the like, a vegetative propagation system for atissue culture seedling of the panacis majoris rhizoma is finally established successfully, the technical basis is laid for the protection and cultivation of the wild resources of the panacis majorisrhizoma, the demand of the seedling, and the establishment of a gene pool, and the problems of low yield, low supply and long natural cultivation period of the wild panacis majoris rhizoma are solved.

The invention discloses a method for arranging a greening layer on the top of a building or a public facility. The method comprises the step of laying a callisia repens lawn with soil or callisia repens blanket without soil on the top of the building or the public facility. Callisia repens is laid on the top of the building or public facility and can be used as a house thermal insulation layer for protecting the building and prolonging the service life of the building; stagnant water drainage load can be lightened, the urban water environment is improved; and urban air can be purified, the noise is lowered, the automobile exhaust is filtered, and the pollution of PM2.5 is lightened.

The invention provides a rapid propagation method for melia toosendan tissue culture. The rapid propagation method for melia toosendan tissue culture comprises the steps of obtaining aseptic melia toosendan leaves, inducing callus tissues, differentiating the callus tissues, inducing rooting, performing acclimatization and transplanting and the like. By means of the rapid propagation method for melia toosendan tissue culture, high-quality seedlings can be cultivated, a cultivation period can be shortened, genetic resources can be preserved, and a basis is provided for melia toosendan development and utilization.

The invention discloses a method for inducing callus of peony petals, applying an MS culture medium that contains 6-BA cytokinin, NAA plant growth regulator, 2, 4-D weedicide and the like to induce callus. Specific culture medium scheme and hormone combination are supplied for explants of peony petals. The optimal material drawn period of the callus is described in the invention. The inductivity of the peony callus is more than 80% and a preferable induction effect is achieved; and the inductivity of the petal callus is higher than 50%.

The invention discloses a rapid propagation method for sapium sebiferum callus seedlings. The method comprises the following steps: 1, taking sapium sebiferum seeds, removing seed coat, disinfecting to obtain explants, inoculating the explants into an MS germination culture medium, and culturing for several days to obtain sterile test-tube plantlets; 2, placing cotyledons of the sterile test-tubeplantlets in a WPM callus culture medium, culturing to obtain callus, culturing the callus in a WPM differential culture medium to obtain multiple shoots, culturing the multiple shoots in a WPM soundseedling culture medium to obtain plants, and culturing the plants in a 1 / 2 WPM rooting culture medium, thereby obtaining rooted seedlings. According to the method disclosed by the invention, lots ofsapium sebiferum seedlings suitable for transplanting are cultivated in a short time, the multiplication coefficient and seedling quality of the sapium sebiferum seedlings are obviously improved, andthe requirements on the sapium sebiferum seedlings in the market are met.

The invention discloses an inducing method for murraya paniculata callus. The inducing method comprises the following steps: S1) pre-treating materials: using 75% ethanol solution for disinfecting mature murraya paniculata seeds for 1-2min, and then using mercury bichloride containing Tween 20 for disinfecting seeds for 6-12min, removing seed coats from the disinfected seeds, and inoculating intoa MS minimal medium for culturing aseptic seedlings; S2) inducing callus: taking blades or root segments of aseptic seedlings as explants, and inoculating into an induction medium for culturing till growing callus. According to the invention, the blades or root segments of aseptic seedlings are taken as explants; the induction medium suitable for callus of blades or root segments is respectively researched; the inductivity of murraya paniculata callus can be increased while the browning rate is reduced; the subsequent proliferation subculture of callus is not influenced; the inducing method can be applied to murraya paniculata seedling propagation, polyploid induction, mutation induction and transgenosis germplasm innovation.

The invention discloses a grass blanket. The grass blanket comprises a modeling framework layer, a shredded coconut blanket layer and a callisia repens blanket layer sequentially from bottom to top, wherein the shredded coconut blanket layer is laid on the modeling framework layer and connected with the modeling framework layer in a fastening manner, and the callisia repens blanket layer is laid on the shredded coconut blanket layer and bound and fixed with the shredded coconut blanket layer. By arranging the modeling framework layer, the shredded coconut blanket layer covering the modeling framework layer, the callisia repens blanket layer covering the shredded coconut blanket layer as well as a landscape device containing the grass blanket, the problems that the stereo modeling cost of plants is high, the use places are limited and different seasonal aspect landscapes are created by replacing seedlings are solved.

The invention provides a method for culturing melia azedarach callus. The method comprises the following steps: pretreating explants; sterilizing the pretreated explant with a 1% sodium hypochlorite solution for 4.5-5.5 min, washing the explants with sterile water, and finally absorbing water; under aseptic conditions, cutting the aseptic explants until the lengths of the aseptic explants are 0.8-1.2 cm, and placing the morphological lower ends of the aseptic explants into a culture flask to carry out induction culture on the callus so as to obtain a target callus. The induction culture mediumcomprises MS+ 1.5 mol / L 6-BA+ 0.5 mol / L NAA+ 25 g / L sucrose+ 7 g / L agar+ 0.8 g / L PVPK-60, and PH=5.8. The method for culturing the melia azedarach callus is good in disinfection effect, high in induction rate and low in callus browning rate, lays a solid foundation for research of tissue culture of melia azedarach, and provides technical support for asexual propagation of the melia azedarach.

The invention provides an in vitro arisaema decipiens culture method. The in vitro arisaema decipiens culture method provided by the invention comprises the following steps: cutting leaf stalks of tissue culture aseptic seedlings of arisaema decipiens to obtain leaf stalk sections; carrying out callus induction culture on the leaf stalk sections to obtain calluses; then culturing the calluses to obtain arisaema decipiens regeneration seedlings. Experiments prove that the arisaema decipiens regeneration seedlings can be successfully obtained by utilizing the in vitro arisaema decipiens culture method, and the method is of great importance to effective protection and utilization of the wild arisaema decipiens resources.

The invention provides a method for hardening and transplanting callus-induced tissue-cultured seedlings of S. martinata, belonging to the technical field of plant tissue culture. The seedling hardening and transplanting method of the callus-induced P. chinensis tissue-cultured seedlings provided by the present invention comprises: taking the callus-induced P. chinensis tissue-cultured seedlings to harden the seedlings for 5 to 10 days to obtain the hardened seedlings, said The temperature for seedling hardening is 15-18°C, the light intensity is 800-1000lx, the light is 5-8h per day, and the seedling hardening humidity is 70-80%; the obtained seedlings after hardening are transplanted into the transplanting medium, Add the nutrient solution to the substrate at the roots of the seedlings. Through the seedling hardening and transplanting process of the present invention, the transplanting survival rate of the callus-induced B. The problem of low survival rate after transplantation of tissue cultured seedlings induced by the callus of Martin chrysanthemum.

The invention discloses a method for arranging a greening layer on the top of a building or a public facility. The method comprises the step of laying a callisia repens lawn with soil or callisia repens blanket without soil on the top of the building or the public facility. Callisia repens is laid on the top of the building or public facility and can be used as a house thermal insulation layer for protecting the building and prolonging the service life of the building; stagnant water drainage load can be lightened, the urban water environment is improved; and urban air can be purified, the noise is lowered, the automobile exhaust is filtered, and the pollution of PM2.5 is lightened.

A disclosed method for utilizing lilium brownie roots to induce callus and embryoid comprises the following steps: 1) roots of lilium brownie aseptic seedling are selected and inoculated to an induction medium for dark culture with the culture room temperature of 23-27 DEG C; 2) embryoids on callus obtained by dark culture are transplanted to a growth rooting medium for culture in light with illumination for 16-20 h every day at a temperature of 23-27 DEG C; and 3) the embryoids are greened, gradually grow into normal plants, and root. By employing the specific culture method and the culture medium provided by the invention, the inductivity of callus induced by lilium brownie roots reaches 100%, all root explants are overgrown with calluses after cultured for about 50 days, the embryoid inductivity is 90% and the plants grow well.

The invention relates to a plant callus culture method and particularly relates to a ranunculus chinensis callus culture method. The ranunculus chinensis callus culture method comprises the following three steps: selecting explants and sterilizing; inducing calluses; amplifying and reproducing the calluses. According to the ranunculus chinensis callus culture method, a lot of the ranunculus chinensis calluses can be obtained in short time, and the effective induction and rapid reproduction of the ranunculus chinensis calluses are realized; the demanding problem of ranunculus chinensis resources is solved; the ranunculus chinensis callus culture method has the characteristics of short production period and rapid reproduction speed, and can be used for extraction and pharmaceuticals industries of effective components of ranunculus chinensis.

The invention relates to a southern vegetable greenhouse roof green grass patch and a production method thereof. The green grass paste comprises a substrate layer, mosses, carbonized garden wastes andcallisia repens; the substrate layer comprises the mosses and the carbonized garden wastes with a weight percentage of 1:1, the mosses and the carbonized garden wastes are evenly mixed, and then pressed to form 1-1.5 cm thick sheet patches; and callisia repens is planted on the substrate. The production method is as follows: step 1: collecting fresh mosses, performing washing and cleaning, and performing drying to make the water content of the mosses to be 15-20%; step 2: crushing dead branches in gardens to be 20-40 mesh, and performing carbonizing; step 3: mixing the mosses in step 1 and the carbonized garden wastes in step 2 according to the ratio of 1:1, and pressing the mixture into 1-1.5 cm thick sheets to form the substrate; and step 4: planting callisia repens on the substrate prepared in step 3, and then covering southern vegetable greenhouse roofs.

The invention discloses a dendrobium seedling raising method, which comprises the following steps: a, culturing a dendrobium stem segment with a node in a culture medium, and inducing an axillary bud; b, when the axillary bud grows to be higher than 1cm, removing the bud, and inducing cluster buds; c, when the cluster buds grow to 1 to 2cm, cutting the cluster buds, performing proliferation and rooting culture on the cluster buds, continuing culturing the original stem segment from which the cluster buds are cut, and inducing calluses; d, after the calluses are formed, cutting and transferring the calluses into the culture medium for continuous culture, and inducing the cluster buds; e, transferring the cluster buds into the culture medium for proliferation culture; f, when the cluster buds grow to 2 to 3cm, transferring the cluster buds into the culture medium for rooting culture. According to the technical scheme, the proliferation number can be effectively increased, so that a large number of high-quality dendrobium seedlings can be produced.

The invention discloses an anti-browning culture medium for elaeagnus mollis tissues and a preparation method of the anti-browning tissue culture medium. The anti-browning culture medium for the elaeagnus mollis tissues comprises a primary culture medium, a bud induction culture medium and a root induction culture medium. According to the invention, through optimizing various components of the culture medium, cytokinin and auxin in the culture medium are adjusted, especially the content of Vc in the culture medium is added and adjusted, the browning rate of callus, aseptic seedlings and elaeagnus mollis seedlings is greatly reduced, the culture medium is favorable for survival, germination and rooting of the elaeagnus mollis tissues, the rooting rate of rooted seedlings is high, the rooting speed is also high, a root system is developed, the lignification of young seedlings is good, and the transplanting survival rate is high. The anti-browning culture medium for the elaeagnus mollis tissues has a simple formula, and is convenient to use and good in effect.

The invention discloses a method for induction of a panax notoginseng purpurin callus. Tender stems, leaves, flowers or anthers of panax notoginseng plants are used as explant, and sterilization, inoculation and induction culture are conducted on the explant to obtain the panax notoginseng purpurin callus; 4-8 h of illumination and 4-8 h of dark treatment are conducted alternately for induction culture; a culture medium for induction culture is MS+0.1-2 mg / L 2,4-D+0.1-2 mg / L BA+0.002-1 mg / L TDZ+30-60 g / L saccharose or MS+0.1-2 mg / L NAA+0.1-2 mg / L BA+0.002-1 mg / L TDZ+30-60 g / L saccharose. By controlling illumination time, the culture medium, moisture, temperature and other conditions, the callus rich in purpurin and high in inductivity is cultured. By shortening the alternation time of illumination and dark treatment, the content of panax notoginseng purpurin is effectively increased; by adding TDZ to the culture medium to be matched with other plant hormones, the inductivity of the panax notoginseng callus is improved remarkably, and formation of purpurin can be achieved easily. The method lays a foundation for mass extraction of purpurin in the panax notoginseng callus.

The invention discloses a roof greening method by using callisia repens, in particular to a growing method for planting callisia repens on roof. The procedures of the method are as follows: first, a water-resistant system, a drainage system and a root resistant layer are arranged on the roof and a matrix layer is paved on the roof; second, grass seeds of the callisia repens is planted on the matrix layer by using any one method of offshoots, broadcasting sowing method, cottage or greensward transplanting and paving method; third, root fixing water is watered and N fertilizer is fertilized once every fifteen days, and lawn is formed within one to two months. As an effective measure for solving the influence of urban development on the entironment and raising the urban afforestation rate, the invention uses the callisia repens on the roof greening, which has a resultful effect on the heat resistance, virescence and disaster prevention of room surface.