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Panacis majoris rhizoma tissue culture and in-vitro regeneration method

A technology of tissue culture and in vitro regeneration, applied in the field of bioengineering, can solve the problems of low yield and high cost of planting ginseng, and achieve the effect of solving the problem of seedlings

Active Publication Date: 2018-11-06
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the above problems, the present invention provides a method, that is, a method for tissue culture and in vitro regeneration of Ginseng Ginseng, which can realize the tissue culture of Ginseng Ginseng Large-scale reproduction, solving the problems of high cost and low yield of pearl ginseng

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Embodiment 1

[0026] (1) Selection and disinfection of explants: Select the rhizomes of Ginseng in the same year, wash the soil under tap water, use a soft brush to dip a small amount of detergent to scrub, rinse with tap water for 2 hours, put it in a beaker and turn it to ultra-clean work On the stage, add 75% alcohol and shake gently for 10 seconds, pour off the alcohol, wash with sterile water twice, add 0.1% mercuric acid solution to cover the explants, shake properly, pour out mercuric acid after disinfection for 15 minutes, and wash with sterile water 6 times, wash and set aside.

[0027] (2) Induction of callus: Cut the sterilized Ginseng rhizome into two halves under aseptic operation, insert it into the callus induction medium in a flat manner, contact the cut surface of the rhizome with the medium, and cultivate it for 20 days , callus tissue began to form on the cut edge of the rhizome. Culture conditions: temperature 25±2°C, relative air humidity 50%-60%, light intensity 2000L...

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Abstract

The invention discloses a panacis majoris rhizoma tissue culture and in-vitro regeneration method. The panacis majoris rhizoma tissue culture and in-vitro regeneration method comprises selecting and disinfecting an explant, inducing callus, differentiating and inducing a bud, inducing rooting and the like. The tissue culture and the clone establishment of the panacis majoris rhizoma are explored to determine the ideal culture conditions for acquiring a sterile material, inducing the callus, differentiating the callus, forming the bud, rooting and the like, a vegetative propagation system for atissue culture seedling of the panacis majoris rhizoma is finally established successfully, the technical basis is laid for the protection and cultivation of the wild resources of the panacis majorisrhizoma, the demand of the seedling, and the establishment of a gene pool, and the problems of low yield, low supply and long natural cultivation period of the wild panacis majoris rhizoma are solved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for tissue culture and in vitro regeneration of ginseng. Background technique [0002] Panax japonicus (Panax japonicus var. major) is a perennial herb of the Araliaceae genus Ginseng. Its rhizome is used as medicine as Panax japonicus. Heat and thirst, consumptive cough, fall injury, joint pain, hemoptysis, hematemesis and traumatic bleeding embolism. The main components of pearl ginseng are saponins, including bamboo ginseng saponins Ⅴ, Ⅳ, Ⅳ a 、Ⅳ a Methyl esters, ginsenosides Rb1, Rc, Rd, Re, Rg1, Rg2 and infarctin R1, R2, etc., among which 4(R)-infarctin R1 has the activity of treating insulin resistance and can significantly reduce the body weight of obese mice. Pharmacological studies have shown that the total saponins of ginseng have anti-inflammatory, central nervous system inhibition, immune function enhancement, anti-cerebral ischemia, anti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 尹进超左应梅张金渝曾琼杨维泽曾祥飞杨天梅李纪潮赵文芹
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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