In vitro arisaema decipiens culture method

A technology of in vitro culture and southern stars, applied in the field of in vitro culture of strange southern stars, can solve the problems of few strange southern stars and no reports on tissue culture

Active Publication Date: 2015-05-27
MINZU UNIVERSITY OF CHINA
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the strange southern star. There is only one

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, strange southern star in vitro propagation method

[0022] 1. Plant material source and disinfection treatment

[0023] Qi Nanxing seeds were collected in Longbang Town, Jingxi County, Guangxi Province. Rinse the collected seeds with tap water for 1 hour, soak them in distilled water for 24 hours, and then sterilize the surface with 75% ethanol for 30 seconds in the ultra-clean workbench, then sterilize with 0.1% mercury liter for 7 minutes, and finally use aseptic Rinse with water 5 times.

[0024] 2. Culture medium and culture conditions

[0025] Using MS medium as the basic medium, the medium was autoclaved at 121°C for 20 minutes. Use sterilized seeds as explants, after inoculation, light for 12 hours a day, place at a temperature of 25±2°C, and light intensity of 30-40mmol·m -2 ·s -1 culture room for cultivation.

[0026] 3. Obtaining sterile vaccines

[0027] About one week or so, the sterilized seeds inoculated on the MS medium began to germi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
Login to view more

Abstract

The invention provides an in vitro arisaema decipiens culture method. The in vitro arisaema decipiens culture method provided by the invention comprises the following steps: cutting leaf stalks of tissue culture aseptic seedlings of arisaema decipiens to obtain leaf stalk sections; carrying out callus induction culture on the leaf stalk sections to obtain calluses; then culturing the calluses to obtain arisaema decipiens regeneration seedlings. Experiments prove that the arisaema decipiens regeneration seedlings can be successfully obtained by utilizing the in vitro arisaema decipiens culture method, and the method is of great importance to effective protection and utilization of the wild arisaema decipiens resources.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an in vitro culture method of the strange southern star. Background technique [0002] Arisaema decipiens, also known as iron lampstand and green-legged lotus, is a plant of the genus Arisaema in the family Araceae. It is distributed in India, Guangxi, Yunnan and other places in mainland China. It grows at an altitude of 880 meters to 3400 meters. In the area, it mostly grows in hillside shrubs, and has not yet been artificially introduced and cultivated. The rhizome of this plant is used as medicine to treat unknown swelling, mastitis, carbuncle, venomous snake bites, bee scorpion stings, spider and mouse bites. In the settlements of the Dong ethnic group, the locals treat breast cancer by externally using the tubers of the strange southern star. At present, there are very few studies on the strange southern star. There is only one report on its chemical composition, and there is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 王俊丽张璐李晓旭马林喜费良丹田璧瑞郭萌彭耀
Owner MINZU UNIVERSITY OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap