A method for hardening and transplanting of callus-induced T.

A technology of callus induction and exposure to martinis, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of low survival rate, reduced survival rate, limited buffer capacity, etc., and reduce transplanting death rate, increase the survival rate, and increase the effect of resistance

Active Publication Date: 2019-11-01
FORESTRY RES INST OF HEILONGJIANG PROVINCE
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the seeds used for seed propagation require a complicated germination process, and the emergence of seedlings is uneven, the initial growth is slow, and the fruit is affected by the size of the year, which makes lilac varieties more flower-seeing types, because most of them are heterozygous genetic types , so seed propagation cannot maintain the typical traits of its variety
[0003] At present, some studies have been done on the in vitro culture of Matinaceae, and a relatively systematic tissue culture regeneration system of Matinaceae has been established by using two methods of bud proliferation and callus tissue. Under the cultivation environment, the root system adapts to the surrounding environment with sufficient water and nutrients. Secondly, the test-tube seedlings are small, have very limited buffering capacity against adverse environments, and have poor resistance. They are directly transplanted into the substrate and placed in the natural environment. It is very easy to cause water shortage and wilt in the environment, which reduces the survival rate; in addition, for P. martinica, the tissue cultured seedlings induced by callus are weaker than those induced by general stem segments, which aggravates the transplanting mortality and makes the lower survival rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Select the mature seeds of S. japonica, clean them with washing powder, and then rinse them with water for 1 hour; place them on an ultra-clean workbench and soak them in 75% ethanol by volume for 30 seconds, and rinse them with sterile water 3 times, air-dried on sterile filter paper, then sterilized with 0.1% mercury liter for 8 minutes, and then rinsed with sterile water 3 times to obtain sterilized seeds;

[0042] (2) Cut the sterilized seeds, insert the hypocotyls into the MS culture medium and cultivate them. The culture conditions are: the culture temperature is 22° C., the light intensity is 2000 lx, the light time is 12 hours per day, and cultured for 10 days. hypocotyl;

[0043] (3) Get 5mm hypocotyls and inoculate them on the callus induction medium that uses MS solid medium as the basic medium and contains 5mg / LBA and 0.5mg / LIBA, at a temperature of 20°C and a light intensity of 2000lx , 12 hours of light per day, cultured for 50 days, and callus tissue...

Embodiment 2

[0048] (1) Select the mature seeds of S. japonica, clean them with washing powder, and then rinse them with water for 2 hours; place them on the ultra-clean workbench and soak them in 75% ethanol for 30 seconds, and rinse them with sterile water 3 times, air-dried on sterile filter paper, then sterilized with 0.1% mercury liter for 8 minutes, and then rinsed with sterile water 3 times to obtain sterilized seeds;

[0049] (2) Cut the sterilized seeds, insert the hypocotyls into the MS culture medium and cultivate them. The culture conditions are: the culture temperature is 20° C., the light intensity is 2000 lx, the light time is 12 hours per day, and cultured for 10 days. hypocotyl;

[0050] (3) Get 6mm hypocotyls and inoculate them on the callus induction medium that uses MS solid medium as the basic medium and contains 5mg / LBA and 0.5mg / LIBA, at a temperature of 20°C and a light intensity of 2000lx , light 12h every day, culture for 40 days, get callus;

[0051] (4) Inocul...

Embodiment 3

[0055] (1) Select the mature seeds of Pamarinica, clean them with washing powder, and then rinse them with water for 1.5 hours; place them on an ultra-clean workbench and soak them in ethanol with a volume percentage of 75% for 30 seconds, and rinse them with sterile water Rinse 5 times, air-dry on sterile filter paper, then sterilize with 0.1% mercuric chloride for 8 minutes, then rinse 3 times with sterile water to obtain sterilized seeds;

[0056] (2) Cut the sterilized seeds, insert the hypocotyls into the MS culture medium and cultivate them. The culture conditions are: the culture temperature is 24°C, the light intensity is 2000lx, the light time is 12h per day, and cultured for 10 days. hypocotyl;

[0057] (3) Get 7mm hypocotyls and inoculate them on the callus induction medium that uses MS solid medium as the basic medium and contains 5mg / LBA and 0.5mg / LIBA, at a temperature of 20°C and a light intensity of 2000lx , 12 hours of light per day, cultured for 60 days, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for hardening and transplanting callus-induced tissue-cultured seedlings of S. martinata, belonging to the technical field of plant tissue culture. The seedling hardening and transplanting method of the callus-induced P. chinensis tissue-cultured seedlings provided by the present invention comprises: taking the callus-induced P. chinensis tissue-cultured seedlings to harden the seedlings for 5 to 10 days to obtain the hardened seedlings, said The temperature for seedling hardening is 15-18°C, the light intensity is 800-1000lx, the light is 5-8h per day, and the seedling hardening humidity is 70-80%; the obtained seedlings after hardening are transplanted into the transplanting medium, Add the nutrient solution to the substrate at the roots of the seedlings. Through the seedling hardening and transplanting process of the present invention, the transplanting survival rate of the callus-induced B. The problem of low survival rate after transplantation of tissue cultured seedlings induced by the callus of Martin chrysanthemum.

Description

technical field [0001] The invention belongs to the technical field of biological tissue culture, and more specifically relates to a method for hardening and transplanting callus-induced callus tissue-cultured seedlings. Background technique [0002] Syringareticulata (Blume), also known as Paomazi and white lilac, is a small deciduous tree of the clove tree in the family Oleaceae. There are about 35 species of lilac trees in the world, mainly distributed in the temperate regions of Asia and Europe, and more than 25 species are produced in China. As an ornamental plant, it is one of the most commonly used tree species in gardens in northern my country because of its luxuriant branches and leaves, large inflorescences, and strong fragrance. In addition, its material is solid and dense, and its structure is uniform; its essential oil content is rich and has a wide range of properties. biological activity; the landscape value and economic value of S. martinica have been paid mo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 舒钰邢亚娟李春明王丹赵学丽王钰婷曹焱
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products