Method for utilizing lilium brownie roots to induce callus and embryoid

A technology of callus and embryoid, which is applied in the field of inducing callus and embryoid by lily root, can solve the problem of no useful roots, etc., and achieve the goal of reducing pollution opportunities, simple cultivation process, and simple induction medium Effect

Inactive Publication Date: 2014-12-10
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The explants used for rapid propagation of lilies in vitro include scales, seeds, bulbils, stem segments, petals, filaments, leaves, anthers, ovaries, pedicels, etc., but there has been no report of using roots as explants so far.

Method used

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  • Method for utilizing lilium brownie roots to induce callus and embryoid
  • Method for utilizing lilium brownie roots to induce callus and embryoid
  • Method for utilizing lilium brownie roots to induce callus and embryoid

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Embodiment 1

[0029] (1) Prepare medium:

[0030] Induction medium: MS medium 1L, sucrose 30000mg / L, agar powder 5000mg / L, and a special composition consisting of 6-BA0.5mg / L, PIC1.0mg / L, hydrolyzed casein 500mg / L.

[0031] Growth and rooting medium: MS medium 1L, BA0.2mg / L, HMI0.2mg / L.

[0032] After preparation, adjust pH=5.4-5.6, sterilize at 121±2°C for 20 minutes at 15-20 pounds, and set aside.

[0033] (2) Select the root of the aseptic lily seedling, inoculate it in the induction medium and carry out dark culture, the temperature is 25±2°C, and callus will be produced in about 30 days (see figure 1 ), gradually producing embryoid bodies (see figure 2 ).

[0034] (3) The embryoid bodies were peeled off in about 50 days, transferred to the growth and rooting medium, cultured under the light, the temperature was 25±2°C, and the light was 16 hours a day, and gradually turned green (see image 3 ).

[0035] (4) grow into rooted normal plants through 1 month cultivation (see Figure...

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Abstract

A disclosed method for utilizing lilium brownie roots to induce callus and embryoid comprises the following steps: 1) roots of lilium brownie aseptic seedling are selected and inoculated to an induction medium for dark culture with the culture room temperature of 23-27 DEG C; 2) embryoids on callus obtained by dark culture are transplanted to a growth rooting medium for culture in light with illumination for 16-20 h every day at a temperature of 23-27 DEG C; and 3) the embryoids are greened, gradually grow into normal plants, and root. By employing the specific culture method and the culture medium provided by the invention, the inductivity of callus induced by lilium brownie roots reaches 100%, all root explants are overgrown with calluses after cultured for about 50 days, the embryoid inductivity is 90% and the plants grow well.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for inducing callus and embryoid bodies by using lily roots. Background technique [0002] Lily is a general term for plants of the genus Lilium (m) in the family Liliaceae. Lily is not only favored by people all over the world for its large flowers, rich colors and fragrant smell, but also has edible and medicinal value. [0003] The traditional propagation method of lilies is mainly based on bulbs, scales, and bulbils, and seeds can also be used for propagation. However, these methods have small reproduction coefficients, especially after multi-generational reproduction, often cause species degradation, and even virus accumulation, which affects the yield and quality of lily, and restricts the large-scale production of lily bulbs and cut flowers. [0004] Lilium tissue culture and plant regeneration can be achieved through the following three pathways: (1) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 周音张建军陈敏敏谢纪红
Owner SHANGHAI ACAD OF AGRI SCI
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