Method for culturing melia azedarach callus

A technology of callus and culture method, which is applied in the field of bioengineering, can solve the problems of neem explant disinfection, primary culture and callus browning treatment, less neem and so on, and achieve good induction effect, high viability, The effect of low pollution rate

Active Publication Date: 2019-05-07
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there are few tissue culture rapid propagation systems for neem, and there are no special research reports on the processes of disinfection, primary culture and callus browning treatment of neem explants.

Method used

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  • Method for culturing melia azedarach callus
  • Method for culturing melia azedarach callus
  • Method for culturing melia azedarach callus

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A kind of culture method of neem callus of the present embodiment, comprises following specific steps:

[0030] (1) Pretreatment of explants:

[0031] The explants were taken as explants of neem leaves from Xinguanzhou Forest Farm, Peiwen Village, Haikou Town, Daguan District, Anqing City, and rinsed with washing powder for 1 minute; then, rinsed with running water for 2 hours until the surface of the leaves No residual dirt; finally, place it in an ultra-clean workbench, soak it with 75% ethanol solution for 30s, pour out the ethanol solution quickly, rinse it with sterile water 5 times, and set aside;

[0032] (2) Disinfection of explants:

[0033] The pretreated neem leaves were sterilized with 1% sodium hypochlorite solution for 5 minutes, rinsed with sterile water for 5 times, and finally blotted dry with sterile filter paper to obtain the sterile explants required for inoculation;

[0034] (3) Culture of callus:

[0035] Under aseptic conditions, the aseptic le...

Embodiment 2

[0037] A kind of culture method of neem callus of the present embodiment, comprises following specific steps:

[0038] (1) Pretreatment of explants:

[0039] The explants were taken as explants from the Xinguanzhou Forest Farm of Peiwen Village, Haikou Town, Daguan District, Anqing City, and were washed with washing powder for 0.8 minutes; then, rinsed with running water for 1.5 hours until the neem There is no residual dirt on the surface of the terminal bud; finally, it is placed in an ultra-clean workbench, and soaked in 75% ethanol solution for 25s, after quickly pouring out the ethanol solution, rinse it with sterile water 4 times, and set it aside;

[0040] (2) Disinfection of explants:

[0041] The pretreated neem terminal buds were sterilized with 1% sodium hypochlorite solution for 4.5 minutes, rinsed with sterile water for 4 times, and finally blotted dry with sterile filter paper to obtain the sterile explants required for inoculation;

[0042] (3) Culture of call...

Embodiment 3

[0045] A kind of culture method of neem callus of the present embodiment, comprises following specific steps:

[0046] (1) Pretreatment of explants:

[0047] Take neem stems from Xinguanzhou Forest Farm, Peiwen Village, Haikou Town, Daguan District, Anqing City as explants, and wash them with washing powder for 1.2 minutes; then, wash them with running water for 2.5 hours until the neem There is no residual dirt on the surface of the stem section; finally, it is placed in an ultra-clean workbench, and soaked in 75% ethanol solution for 35s, after pouring out the ethanol solution quickly, rinsed with sterile water 6 times, and set aside;

[0048] (2) Disinfection of explants:

[0049] Sterilize the pretreated neem stem section with 1% sodium hypochlorite solution for 5.5 minutes, rinse it with sterile water for 6 times, and finally dry it with sterile filter paper to obtain the sterile explant required for inoculation;

[0050] (3) Culture of callus:

[0051] Under aseptic con...

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Abstract

The invention provides a method for culturing melia azedarach callus. The method comprises the following steps: pretreating explants; sterilizing the pretreated explant with a 1% sodium hypochlorite solution for 4.5-5.5 min, washing the explants with sterile water, and finally absorbing water; under aseptic conditions, cutting the aseptic explants until the lengths of the aseptic explants are 0.8-1.2 cm, and placing the morphological lower ends of the aseptic explants into a culture flask to carry out induction culture on the callus so as to obtain a target callus. The induction culture mediumcomprises MS+ 1.5 mol / L 6-BA+ 0.5 mol / L NAA+ 25 g / L sucrose+ 7 g / L agar+ 0.8 g / L PVPK-60, and PH=5.8. The method for culturing the melia azedarach callus is good in disinfection effect, high in induction rate and low in callus browning rate, lays a solid foundation for research of tissue culture of melia azedarach, and provides technical support for asexual propagation of the melia azedarach.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for cultivating neem callus. Background technique [0002] Melia azedarach is a deciduous tree belonging to Meliaceae, also known as Kuling and Mori. As a native tree species, neem is more common in the provinces south of the Yellow River in my country. The tree can not only absorb toxic and harmful gases such as sulfur dioxide and hydrogen fluoride, but also is an expert insecticide, which can prevent and control serious agricultural pests. It is called a non-polluting plant insecticide. Neem grows fast, has excellent material, insect repellent, and rot resistance. Its roots, bark, flowers, and fruits can be used as medicine. Saline-alkali soil vegetation restoration tree species. At present, neem mostly adopts the method of sowing for seedling breeding, but this method has a large workload, not only time-consuming and labor-intensive, but also slow propagatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 项艳罗霜霜胡文芳何婷王雅梅
Owner ANHUI AGRICULTURAL UNIVERSITY
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