A kind of induction method of notoginseng purple pigment callus

A callus, violet pigment technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, to achieve the effects of increasing the induction rate, increasing the content and facilitating the formation of violet pigment

Active Publication Date: 2018-04-24
文山苗乡三七科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are currently no relevant literature a

Method used

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  • A kind of induction method of notoginseng purple pigment callus
  • A kind of induction method of notoginseng purple pigment callus
  • A kind of induction method of notoginseng purple pigment callus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for inducing a notoginseng purple pigment callus, comprising the following steps:

[0023] Select the young and tender stems, leaves, and flowers of healthy Panax notoginseng plants as explants, rinse them with water for 5 times, sterilize them with 70% alcohol for 10 seconds, rinse them with sterile water for 3 times, and then wash the explants. The implant was placed in 0.1% mercuric chloride, soaked for sterilization, rinsed with sterile deionized water for 6 times, and set aside.

[0024] Cut the sterilized stems, leaves and flowers into 0.5cm in a sterile environment 2 block explants; under sterile conditions, open the tissue culture bottle near the flame of the alcohol lamp, burn the bottle mouth on the flame, inoculate the cut explants on the medium, each bottle of medium Inoculate 3 to 5 explants.

[0025] Induction culture was carried out in the medium of MS+0.1mg / L 2,4-D+0.1mg / L BA+0.002mg / L TDZ+30g / L sucrose, and the culture period was 4-5 weeks. ...

Embodiment 2

[0028] A method for inducing a notoginseng purple pigment callus, comprising the following steps:

[0029] Select the young and tender stems, leaves and flowers of healthy Panax notoginseng plants as explants, rinse them with water for 6 times, sterilize them with 70% alcohol for 10 seconds, rinse them with sterile water for 3 times, and then rinse the explants with 70% alcohol. The implant was placed in 0.1% mercuric chloride, soaked for sterilization, rinsed with sterile deionized water 8 times, and set aside.

[0030] In a sterile environment, cut the sterilized stems, leaves, and flowers into 0.5cm2 block explants; under sterile conditions, open the tissue culture bottle near the flame of the alcohol lamp, and burn the bottle mouth on the flame At once, the cut explants were inoculated on the culture medium, and 5 explants were inoculated in each bottle of culture medium.

[0031] The induction medium is MS+2mg / L 2,4-D+2mg / L BA+1mg / L TDZ+60g / L sucrose.

[0032] Among the...

Embodiment 3

[0034] A method for inducing a notoginseng purple pigment callus, comprising the following steps:

[0035] Select healthy anthers of Panax notoginseng as explants, wash the Sanqi flowers with clear water 5 times, sterilize them with 70% alcohol for 10 seconds, and then place the washed explants in 0.1% mercury chloride. Soak for sterilization, rinse with sterile deionized water for 6 times, and set aside.

[0036] Strip off the anthers on sterile filter paper; under sterile conditions, open the tissue culture bottle near the flame of the alcohol lamp, burn the mouth of the bottle on the flame, and inoculate the anthers into the tissue culture bottle, 15 capsules per bottle;

[0037] Induction culture was carried out in the medium of MS+0.1mg / L NAA+0.1mg / L BA+0.002mg / L TDZ+30g / L sucrose.

[0038] Wherein, the light control is 6 hours of light conditions and 6 hours of dark conditions alternately, the light intensity is 1600lx; the temperature: 22°C; the relative air humidity: ...

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Abstract

The invention discloses a method for induction of a panax notoginseng purpurin callus. Tender stems, leaves, flowers or anthers of panax notoginseng plants are used as explant, and sterilization, inoculation and induction culture are conducted on the explant to obtain the panax notoginseng purpurin callus; 4-8 h of illumination and 4-8 h of dark treatment are conducted alternately for induction culture; a culture medium for induction culture is MS+0.1-2 mg/L 2,4-D+0.1-2 mg/L BA+0.002-1 mg/L TDZ+30-60 g/L saccharose or MS+0.1-2 mg/L NAA+0.1-2 mg/L BA+0.002-1 mg/L TDZ+30-60 g/L saccharose. By controlling illumination time, the culture medium, moisture, temperature and other conditions, the callus rich in purpurin and high in inductivity is cultured. By shortening the alternation time of illumination and dark treatment, the content of panax notoginseng purpurin is effectively increased; by adding TDZ to the culture medium to be matched with other plant hormones, the inductivity of the panax notoginseng callus is improved remarkably, and formation of purpurin can be achieved easily. The method lays a foundation for mass extraction of purpurin in the panax notoginseng callus.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for inducing a notoginseng purple callus. Background technique [0002] Panax notoginseng is a plant of the genus ginseng of Araliaceae, known as "gold does not change", "sacred grass of the south", "king of ginseng", etc. It is a rare and precious Chinese medicinal material unique to Yunnan and a bright pearl in Chinese traditional medicine. Panax notoginseng purple pigment is a compound present in the cell fluid of the stems, leaves, flowers and roots of the Panax notoginseng plant, which makes the plant appear red, purple, blue and other different colors. [0003] The essence of Panax notoginseng purple pigment is anthocyanin, which is a group of compounds that can show red in flavonoids-a class of substances based on flavone cores. Because of its unique functionality, it is used to remove free radicals in the body, proliferate lutein, anti-tumor, anti-cancer, anti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 陈中坚王勇魏富刚余育启
Owner 文山苗乡三七科技有限公司
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