Establishing method of Gleditsia vestita Chun et Howex B.G.Li.tissue culture regeneration system

A technology of acacia follicle and tissue culture, which can be applied in the directions of plant regeneration, horticultural methods, botanical equipment and methods, etc., and can solve problems such as rare reports and the like

Inactive Publication Date: 2016-07-20
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

In order to protect this rare tree species and increase its population, domestic scholars have conducted a...

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  • Establishing method of Gleditsia vestita Chun et Howex B.G.Li.tissue culture regeneration system
  • Establishing method of Gleditsia vestita Chun et Howex B.G.Li.tissue culture regeneration system
  • Establishing method of Gleditsia vestita Chun et Howex B.G.Li.tissue culture regeneration system

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific embodiments, rather than limiting the present invention.

[0030] 1 Materials and methods

[0031] 1.1 Materials

[0032] Some of the mature seeds of the native species of Acacia velvet were picked from near Guangji Temple in Nanyue Nature Reserve, Hengshan City, Hunan Province (27°17′55.26″N; 114°42′15.59″E; 950m above sea level), and brought back to the laboratory for experimentation. Kneading, peeling, washing the seeds, water selection, sorting, natural air drying, and keeping them in a cool and dry place indoors for later use.

[0033] 1.2 Method

[0034] 1.2.1 Disinfection of explants and cultivation of sterile seedlings

[0035] The collected acacia seeds were treated with 70% alcohol on the ultra-clean workbench for 30 seconds, then sterilized with 0.1% mercuric chloride for 30 minutes, and washed with sterile water for 5 times for later use. Inoculate the sterilized seeds on ...

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Abstract

The invention discloses an establishing method of a Gleditsia vestita Chun et Howex B.G.Li.tissue culture regeneration system. The rapid propagation system of Gleditsia vestita Chun et Howex B.G.Li.tissue culture is established by taking cotyledons of aseptic seedlings of Gleditsia vestita Chun et Howex B.G.Li. as explants through callus inducement, adventitious bud proliferation, rooting and acclimatizating and transplanting. The results show that: the cotyledons of the Gleditsia vestita Chun et Howex B.G.Li.simultaneously induce callus and buds on a MS+6-BA3.0mg/L+KT0.4mg/L+IAA0.3mg/L culture medium, and the average induction rate reaches 90.5%; the best culture medium of adventitious bud proliferation is 1/2MS+6-BA1.0mg/L +IBA0.3mg/L+NAA0.2mg/L, and the proliferation multiple is 4.0; under the condition of MS+NAA0.5mg/L+IAA0.25mg/L, the rooting rate is up to 100%; plants after transplanting are good in growth vigor, and the survival rate is over 90%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for establishing a tissue culture regeneration system of Gleditsiavestita Chunet Howex B.G.Li. Background technique [0002] Gleditsiavestita Chunet Howex B.G.Li., also known as Gleditsiavestita Chunet Howex B.G.Li., is a unique species in Hunan and is a national second-class protected plant. Wild velvety acacia is even rarer than the silver fir known as the "Panda in the Plant Kingdom". There are only 4 native species left at the foot of Nanyue Mountain, and one of them has been hollow and decayed. From the taxonomical point of view, Acacia tomentosa is a relatively primitive species in the legume family, and it is of great value in the study of the evolutionary classification of the entire legume family. At the same time, the acacia tomentosa also has high economic value: the fruit is rich in pancreatic saponin, which can be used as a detergent for silk a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘克明田学辉
Owner HUNAN NORMAL UNIVERSITY
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