Breeding method for high-yield black-skin chicken strain

A breeding method and technology for black-skinned chickens, applied in animal husbandry and other directions, can solve the problems of the size of the expansion group, the cumbersome test-crossing process, and the long cycle, so as to speed up the breeding process, overcome the cumbersome test-crossing process, and achieve high production performance. Effect

Active Publication Date: 2014-02-12
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the test cross process is cumbersome, the cycle is l

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  • Breeding method for high-yield black-skin chicken strain

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Embodiment 1

[0030] The breeding method of high-yield type black-skinned chicken strain in the present embodiment, comprises the following steps:

[0031] (1) Using silky silkie pure line cock as the male parent and Bailai aircraft carrier chicken as the female parent to carry out crossbreeding to produce F1 generation chickens;

[0032] (2) The F1 generation is self-crossed to obtain the F2 generation, and the F2 generation black-skinned chicken is selected;

[0033] (3) Determine the relative copy number of the EDN3 gene of the F2 generation of black-skinned chickens by fluorescent quantitative PCR, and determine the genotype of the F2 generation of black-skinned chickens, specifically including the following steps:

[0034] ① Collection of samples

[0035] Take blood from the black-skinned chicken wings selected from the F2 generation, add 1 / 3 anticoagulant, and extract blood DNA by proteinase K method;

[0036] ② DNA sample concentration adjustment

[0037] Use a micro-spectrophotom...

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Abstract

The invention discloses a breeding method for a high-yield black-skin chicken strain and belongs to the technical field of molecular breeding. The genotype of a black-skin site of a chicken is authenticated with a molecular marker-assisted selection method, so that the genotype of the site can be quickly determined, and the shortcomings of complicated testcross, long generation interval and the like in conventional breeding are overcome. The method disclosed by the invention is used for producing the high-yield black-skin chicken strain of which the genotype is FmFm, so that the generation interval is shortened, the breeding progress is accelerated, and the breeding cost is reduced; furthermore, the egg laying performance of the strain is high, and the nutritional value of eggs is high; the black-skin characters of culled hens are uniform, and carcasses are more attractive. Furthermore, the strain is used as a male parent hybridized with high-laying-rate chickens, so that black-skin chickens with higher production performance can be produced.

Description

technical field [0001] The invention specifically relates to a method for cultivating a high-yield black-skinned chicken strain, and belongs to the technical field of molecular breeding. Background technique [0002] Black-bone chicken is a well-known medicinal poultry at home and abroad. It was recognized as a standard breed internationally in 1874. Black-bone chicken and black-bone eggs have special medicinal, nutritional and ornamental values. But the egg production performance of black-bone chicken is low. The laying performance of foreign high-yield laying hens is very good. In order to improve its production performance, breeders often need to introduce bloodlines of high-yield laying hens in breeding practice, which will cause the separation of black skin traits, and then affect the price of culled hens. In order to maintain the black skin trait of culled hens while improving production performance, it is very important to determine the genotype of the black skin lo...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 康相涛田亚东杨朋坤韩瑞丽王丹丹李转见孙桂荣蒋瑞瑞吕世杰闫峰宾王彦彬刘小军李国喜黄艳群郭晗许殿明李孝法索智勇
Owner HENAN AGRICULTURAL UNIVERSITY
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