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Extraction and detection method of opioids in hair

A technology of extraction method and detection method, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of not meeting timeliness requirements, long acid hydrolysis time, high price, etc., to improve detection sensitivity and accuracy, and method specificity. Strong, easy-to-use effects

Active Publication Date: 2015-07-29
广州正孚检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The condition of acid hydrolysis method is relatively mild, and the release efficiency is high, but this method still has the disadvantage that the acid hydrolysis time is too long and does not meet the timeliness requirements, such as "LC-MS / MS analysis of morphine alkaloids in biological samples" The hair was soaked overnight in 0.1mol / L HCl solution in a 45°C water bath (Journal of Forensic Medicine, February 2006, Vol.22, No.1)
Alkaline hydrolysis releases the drug completely, but the conditions are severe; enzymatic hydrolysis has a wide range of applications and high release efficiency, but is expensive

Method used

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  • Extraction and detection method of opioids in hair
  • Extraction and detection method of opioids in hair
  • Extraction and detection method of opioids in hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In this example, opioid standard substances such as morphine, monoacetylmorphine, codeine, and meperidine were added to negative hair, and were analyzed by GC / MS-SIM after compound solution pretreatment, solid phase extraction, and derivatization. Prepare standard supplement samples with concentrations of 2000pg / mg and 30000pg / mg respectively. Prepare 6 samples for each concentration on the same day, measure the ratio of each component to the peak area of ​​the internal standard, calculate the concentration and CV value, and determine the recovery rate and intra-day detection precision of the method; prepare these two concentrations separately for 5 consecutive days The standard is added to the sample, the ratio of each component to the internal standard peak area is measured, and the CV value is calculated to determine the day-to-day detection precision and accuracy of the method. Please see Table 1 and Table 2 for the experimental results.

[0037] 1. Experimental st...

Embodiment 2

[0056] This example is an example of the detection experiment of opioids such as morphine, monoacetylmorphine, codeine and meperidine in the hair of 6 drug abusers. MS-SIM analysis, the experimental results are shown in Table 3.

[0057] 1. Experimental steps:

[0058] (1) Weigh about 40 mg of the hair of the abuser, wash it with 3 mL of detergent, water, and acetone, cut it into 1.0 mm pieces, and grind it with liquid nitrogen to obtain the ground hair powder;

[0059] (2) Accurately weigh 30 mg of the abuser's hair powder after grinding, and add 2 mL of hair compound treatment solution (the specific components are 0.5 mol / L phosphate buffer solution, 3% bovine serum albumin, 1% Tween 20 , and its pH value was adjusted to 4.0), adding an internal standard, ultrasonicating at 75°C for 2 hours, and centrifuging to obtain a supernatant;

[0060] (3) Equilibrate the solid-phase extraction column with 2mL of methanol and 2mL of 0.1mol / L phosphate buffer in sequence, and inject t...

Embodiment 3

[0084] In this example, opioid standard substances such as morphine, monoacetylmorphine, codeine, and meperidine were added to negative hair, and after compound solution pretreatment, solid phase extraction, and derivatization, GC / MS-SIM analysis was performed to determine the method The recovery rate, the experimental results are shown in Table 4.

[0085] 1. Experimental steps:

[0086] (1) with embodiment 1;

[0087] (2) Accurately weigh 30 mg of ground negative hair powder respectively, add 2 mL of hair compound treatment solution (the specific components are 0.5 mol / L phosphate buffer solution, 0.5% bovine serum albumin, 5% Tween 20, And its pH value is adjusted to 4.0), adding an internal standard, sonicating at 80°C for 3.0 hours, and centrifuging to obtain a supernatant;

[0088] (3) Equilibrate the solid-phase extraction column with 2mL methanol and 2mL 0.1mol / L phosphate buffer in sequence, and inject the above-mentioned hair pretreatment supernatant, and use 2mL w...

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Abstract

The invention provides an extraction and detection method of opioid matters in hair. The method comprises the following steps of extraction and purification of hair. The extraction method further comprises a derivation step. The invention further provides a method for detecting the opioid matters in hair by adopting a gas chromatograph-mass spectrometer or a liquid chromatograph mass spectrometer. Compared with conventional hair poison residue detection methods, the method provided by the invention is very mild but can enrich opioid matters effectively and effectively remove various impurity interferents in a hair detection material, has higher sensitivity and accuracy, and can be used by law-enforcing departments, inspection departments and drug test mechanisms of government.

Description

technical field [0001] The invention belongs to the technical field of biomedicine detection, and in particular relates to an extraction method and a detection method of opioids in hair. Background technique [0002] Drugs and abuse of addictive drugs endanger human health and social security, and have always been the targets of severe crackdown by the state according to law. The detection methods of human drug residues are very important to assist law enforcement. Hair analysis has unique advantages such as easy material collection, easy storage, long detection time limit, comprehensive information on drug abuse history, and easy repeated sampling. It has been widely used in the fields of forensic toxicology, clinical toxicology, occupational medicine, and doping control. The evidentiary nature of the analysis of common drugs of abuse in hair has been recognized by courts in most countries. [0003] The composition of hair is complex and there are a lot of interfering subs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 陈园园刘晓云王继华
Owner 广州正孚检测技术有限公司
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