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Irradiation degradation treatment method for fumitremorgin and ochratoxin

A technology of ochratoxin and fumonisin, applied in the field of analytical chemistry, can solve the problems of unreported degradation products and achieve good environmental protection effect

Inactive Publication Date: 2014-03-19
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for effective treatments against bacterial diseases like those caused by Bacillus cerevisiae (Bcc), which causes spoilage during storage and distribution processes. It also prevents damage from harmful microorganisms that may affect crops grown under these conditions. By exposing certain types of organic materials containing BcAg protein called antibodies, this method helps prevent growth of unwanted germ cells while reducing their impact upon healthcare workers who handle them.

Problems solved by technology

This patents discuss various methods used to measure certain substances called volatile tetracycline (TT) or fulminatechin gallotoxin (FUM). These techniques involve extracting these compounds from plant material with specific solvents like acids such as hydrochloric acid followed by gaschromatographic separation into different fractions before quantifying them individually. However, there may exist other chemical components present during processing procedures leading to false positives. Therefore, this technical problem addressed by the patented technology described in the patents involves identifying those chemical components involved in producing these contaminated materials without affecting their quality control requirements.

Method used

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  • Irradiation degradation treatment method for fumitremorgin and ochratoxin
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  • Irradiation degradation treatment method for fumitremorgin and ochratoxin

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Embodiment Construction

[0036] Prepare standard solutions for the standard samples of fumonisin FB1 and ochratoxin OTA, and obtain the standard curve of the toxins, see figure 1 with figure 2 :

[0037] FB1: Y=-4407.4+35124.6*X, R 2 =0.9970, OTA: Y=116009+673499*X, R 2 =0.9979;

[0038] The standard solutions of different concentrations were irradiated with 3-9kGy gamma rays respectively, and the content of the toxin was detected. The unirradiated standard solution corresponding to each toxin was used as a control sample. The response value of the toxin is shown in Table 1. The results in Table 3 show that the irradiation treatment of 3-9kGy has a degradation effect on FB1, and at 9kGy, the degradation rates of FB1 with a concentration of 0.8mg / mL, 10.0ug / mL, 1.0ug / mL and 50ng / mL are 22.5% respectively. %, 51.0%, 59.0% and 64.8%; 3-9kGy irradiation treatment can degrade OTA, but the effect is not obvious. Since in the dose range of 3-9kGy, the degradation effect of the standard solution with t...

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Abstract

The invention relates to an irradiation degradation treatment method for fumitremorgin and ochratoxin. The irradiation degradation treatment method comprises the following steps: (1) preparing standard solutions of standard products of the fumitremorgin FB1 and ochratoxin OTA; carrying out irradiation treatment on the standard solutions in an irradiation range of 0kGy-200kGy; determining the content of the FB1 and the OTA by adopting a liquid chromatogram-tandem mass spectrometry; (2) pre-treating a poisoned sample after irradiating in an irradiation range of 0kGy-9kGy; determining degradation effects on the FB1 and the OTA by adopting the liquid chromatogram-tandem mass spectrometry; and (3) selecting the FB1 and OTA standard solutions with the highest concentration as a sample and carrying out detection and analysis on a degraded product by using the liquid chromatogram-tandem mass spectrometry after irradiating in the irradiation range of 0kGy-200kGy. According to the irradiation degradation treatment method for the fumitremorgin and the ochratoxin, researches and applications of FB1 and OTA degraded products which are lack of irradiation degradation are solved; the degradation effect is good and the environment-friendly detoxification treatment of mildewed agricultural product wastes is realized.

Description

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Claims

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Application Information

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Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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