Dna probe library for hybridization with bcr or abl gene and method for enriching bcr-abl gene fragments using it

Abstract

The invention provides a DNA (Deoxyribonucleic Nucleic Acid) probe library for hybridization with a BCR or ABL gene and a method for enriching BCR-ABL gene segments. The DNA probe library comprises one or more DNA probes capable of being hybridized with the BCR or ABL genes. The invention also provides a method for enriching BCR-ABL gene segments by using the DNA probes. On this basis, the invention also provides a method for detecting whether the mutation of the BCR-ABL gene or the gene structure thereof exists. The method provided by the invention is capable of obtaining the gene segments through enrichment by thousands of times; the method can be applied to the next generation sequencing technology to detect whether the mutation of the BCR-ABL gene or the gene structure thereof exists, including single base mutation, mRNA (messenger Ribose Nucleic Acid) deletion or amplification, mRNA structure transversion and mRNA splicing change.

Description

technical field [0001] The present invention relates to the field of gene detection. Specifically, the present invention relates to a method for enriching and extracting BCR-ABL gene fragments. Detection of BCR-ABL gene and its gene structure mutation. Background technique [0002] DNA sequencing technology is undergoing drastic changes. Its outstanding feature is the observation and analysis of many sites at the same time (massively parallel), so as to gradually achieve a substantial increase in sequencing throughput. The cost of sequencing each base in the original data a sharp decline. Based on this, previously unattainable luxury activities (such as personal gene sequencing, metagenomics research) have gradually become more and more feasible. Especially with the development of science, because traditional Sanger sequencing can no longer fully meet the needs of research, next-generation sequencing technology (Next-generation sequencing technology, Next-generation sequen...

AI Technical Summary

Problems solved by technology

[0018] Aiming at the above-mentioned technical problems, the present inventor has developed a method for capturing BCR-ABL gene fragment sequences based on hybridization selection through a large number of experiments. In the case that the subject has the BCR-ABL gene, this method can obtain thousands of times enriched BCR-ABL gene fragments, the enriched BCR-ABL gene fragment samples can be selectively applied to various gene detection techniques, especially the next generation sequencing technology can be applied to gene mutations, deletions, increases, and Detection of inversion etc.

Images