A kind of citrus peel residue organic compound fertilizer and preparation method thereof
A technology of citrus peel and compound fertilizer, which is applied in the field of harmless treatment of citrus peel
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Embodiment 1
[0054] The preparation of embodiment 1 liquid microbial bacterial agent
[0055] (1) Preparation of Aspergillus niger CGMCC NO.7927 expansion culture fermentation broth
[0056] The well-preserved Aspergillus niger CGMCC No.7927 slant strains were activated by the strains and the first-, second-, and third-level liquid seeds and seed tanks were gradually expanded to obtain liquid seeds, which were inserted into the fermenter with 6% inoculum. The culture temperature is 30°C, the stirring speed is 400rpm, the ventilation rate (V / V) is 1:2, and the culture time is 12h; then slowly cool down to 12°C at a cooling rate of 2°C / h, and cultivate at a constant temperature for 18h; continue to cool at a rate of 2°C / h Slowly lower the temperature to 3°C. At this time, add the liquid seeds to the fermenter with 4% inoculation amount, and cultivate at a constant temperature for 24 hours; finally, slowly raise the temperature to 12°C at a heating rate of 2°C / h, and cultivate at a constant t...
Embodiment 2
[0071] A preparation method of citrus peel residue organic compound fertilizer, comprising the steps of:
[0072] (1) Citrus peel for acid reduction
[0073] Take citrus peels with 50% of the total mass of organic compound fertilizer raw materials, crush them to a particle size of 3mm, add 0.5% of citrus mass by acid-reducing yeast, and ferment at room temperature for 72 hours, stirring once every 12 hours, until the pH value of citrus peels drops to 6;
[0074] The citrus peel residue is the citrus waste residue after industrialized juicing, with a moisture content of 60% and a pH value of 3.8;
[0075] The acid-reduced yeast is composed of the expansion culture fermentation liquid of Pichia pastoris, fission yeast and wine yeast; the concentration of yeast cells in the expansion culture fermentation liquid of Pichia pastoris, fission yeast and wine yeast is respectively 9.0×10 8 pcs / mL, 6.0x10 8 / mL and 8.0x10 7 Individual / mL; The mixed mass ratio of Pichia pastoris, fiss...
Embodiment 3
[0097] A preparation method of citrus peel residue organic compound fertilizer, comprising the steps of:
[0098] (1) Citrus peel for acid reduction
[0099] Take citrus peels with 55% of the total mass of organic compound fertilizer raw materials, crush them to a particle size of 4 mm, add 0.8% of the citrus mass by acid-reducing yeast and ferment at room temperature for 36 hours, and stir once every 12 hours until the pH value of the citrus peels drops to 6;
[0100] The citrus peel residue is the citrus waste residue after industrialized juicing, with a moisture content of 70% and a pH value of 3.8;
[0101] The acid-reduced yeast is composed of the expansion culture fermentation liquid of Pichia pastoris, fission yeast and wine yeast; the concentration of yeast cells in the expansion culture fermentation liquid of Pichia pastoris, fission yeast and wine yeast is respectively 9.0×10 8 pcs / mL, 5.0x10 8 -6.0x10 8 / mL and 7.0x10 7 -8.0x10 7 Individual / mL; The mixed mass r...
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