Serum amyloid A (SAA) protein and encoding gene and application thereof

A serum starch and protein technology, applied in the fields of biochemistry and molecular biology, can solve problems such as yield fluctuations, high risk of oyster farming, and lack of disease detection methods

Active Publication Date: 2014-06-04
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of marine shellfish in China is mainly based on traditional breeding and breeding, at the expense of area and energy consumption. The output fluctuates due t

Method used

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  • Serum amyloid A (SAA) protein and encoding gene and application thereof
  • Serum amyloid A (SAA) protein and encoding gene and application thereof
  • Serum amyloid A (SAA) protein and encoding gene and application thereof

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Embodiment 1

[0013] 1. RNA extraction and reverse transcription

[0014] a). After adding Trizol to Hong Kong giant oyster cells or tissues, grind them with a tissue grinder, and place them at room temperature for 5 minutes to fully lyse them.

[0015] b). Centrifuge at 12,000rpm for 5min and discard the precipitate.

[0016] c). Add chloroform to 200ul chloroform / ml Trizol, vortex and mix well, and place at room temperature for 15min.

[0017] d). Centrifuge at 12,000 g for 15 min at 4°C.

[0018] e). Aspirate the upper aqueous phase into another centrifuge tube.

[0019] f). According to 0.5ml isopropanol / ml Trizol, add isopropanol and mix well, and place at room temperature for 5-10min.

[0020] g). Centrifuge at 12,000g at 4°C for 10 minutes, discard the supernatant, and sink the RNA to the bottom of the tube.

[0021] h). Add 75% ethanol to 1ml 75% ethanol / ml Trizol, shake the centrifuge tube gently, and suspend the precipitate.

[0022] i). Centrifuge at 8,000g at 4°C for 5 minu...

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Abstract

The invention discloses a serum amyloid A (SAA) protein and an encoding gene and an application thereof. The serum amyloid A protein is characterized in that a nucleotide sequence of the serum amyloid A protein is as showed in the 148-567th basic groups of SEQ ID NO.1, and the amino acid sequence of the serum amyloid A protein is as shown in SEQ ID NO.2. The gene is firstly discovered in a mollusk, and at present, the gene is discovered in vertebrates and is only discovered in an echinodermata in invertebrates. The quantitative PCR experiment proves that the oyster SAA is remarkably up-regulated after bacterial infection and is similar to a mammal, and thus the gene is utilized as a gold standard for detecting the oyster physical condition. The gene can monitor the physical condition of the oyster at any time by detection, a positive accurate caution signal is provided for the oyster disease prevention, and the expansion of a disaster is prevented. The application of the technology has the advantages that the germplasm innovation capability of the cultivated shellfish is further improved, and the efficient sustainable development of an industry is supported.

Description

Technical field: [0001] The invention belongs to the field of biochemistry and molecular biology, and in particular relates to a serum amyloid A and its encoding gene and application. Background technique: [0002] As the largest shellfish farming country in the world, shellfish farming in my country has become one of the pillars of the coastal mariculture industry. my country's annual shellfish production accounts for more than 60% of the world's total shellfish production. According to the statistics of the Ministry of Commerce in 2012, in 2011, the output of marine shellfish farming in my country was 11.796 million tons, of which 3.7563 million tons of oysters, with an economic value of more than 20 billion yuan, accounting for more than 30% of the output of marine shellfish farming. Therefore, oysters The aquaculture of China occupies a pivotal position in the economic and social development of my country's coastal areas, and has huge development potential. However, the...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/775C12Q1/68
Inventor 向志明瞿符发肖述喻子牛
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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