A kind of oyster cell apoptosis gene smac gene and its application in the preparation of pathological detection and diagnostic reagents

Abstract

The invention discloses an oyster cell apoptosis gene SMAC gene and an application thereof in the preparation of a pathology detecting and diagnostic reagent. The invention discovers the symbolic geneSMAC gene of the cell apoptosis of the species, and the nuclear antigen sequence is shown as from the 50th bp to the 1006th bp of the SEQ ID NO.1. The amino acid sequence (the amino acid sequence isshown in the SEQ ID NO. 2) of the gene coding has high species specificity, the similarity of the amino acid sequence in ostrea is 60%-88%, the evolution relationship is close, and the similarity withthe homologous proteins of other species is 30% or below, so that the evolution relationship is far. The quantitative polymerase chain reaction (PCR) experiment shows that the SMAC gene is up-regulated after the infection of vibrio alginolyticus and staphylococcus haemolyticus, can be used as a standard for detecting the apoptosis of the oyster cells, provides a positive and accurate warning signal for the prevention of oyster diseases, and prevents the expansion of the disaster.

Description

Technical field: [0001] The present invention relates to the molecular mechanism of shellfish immunity and cell apoptosis, and more specifically relates to a SMAC (secondmitochondria-derived activator of caspases) screened from Hong Kong giant oyster (Crassostrea hongkongensis) blood cell subtractive hybridization library stimulated by Vibrio The nucleotide sequence and amino acid sequence of the gene, its preparation method and the application of the gene. Background technique: [0002] my country is the largest oyster farming country in the world, and according to statistics, its annual output accounts for more than 80% of the world's total output. According to the 2015 China Fishery Statistical Yearbook, the oyster farming area reached 141,500 hectares in that year, and the output was about 4.5734 million tons. Therefore, oyster farming occupies a pivotal position in my country's coastal economic and social development and is an important pillar industry; The Hong Kong gi...

AI Technical Summary

Problems solved by technology

The development of marine shellfish in China is mainly based on traditional farming, which has a large farming area and high energy consumption. The output fluctuates due to changes in various conditions. Moreover, due to the lack of effective disease detection methods, with the expansion of the farming scale, the oyster Breeding risks are also increasing

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