A kind of oyster cell apoptosis gene smac gene and its application in the preparation of pathological detection and diagnostic reagents
An oyster and gene technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of lack of disease detection methods, production fluctuations, and high risks of oyster farming.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] 1. RNA extraction and reverse transcription
[0033] a). After adding Trizol to Hong Kong giant oyster cells or tissues, grind them with a tissue grinder, and place them at room temperature for 5 minutes to fully lyse them.
[0034] b). Centrifuge at 12,000rpm for 5min and discard the precipitate.
[0035] c). Add chloroform to 200ul chloroform / ml Trizol, vortex and mix well, and place at room temperature for 15min.
[0036] d). Centrifuge at 12,000 g for 15 min at 4°C.
[0037] e). Aspirate the upper aqueous phase into another centrifuge tube.
[0038] f). According to 0.5ml isopropanol / ml Trizol, add isopropanol and mix well, and place at room temperature for 5-10min.
[0039] g). Centrifuge at 12,000g at 4°C for 10 minutes, discard the supernatant, and sink the RNA to the bottom of the tube.
[0040] h). Add 75% ethanol to 1ml 75% ethanol / ml Trizol, shake the centrifuge tube gently, and suspend the precipitate.
[0041] i). Centrifuge at 8,000g at 4°C for 5 minu...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com