Rat pancreatic duct epithelioid stem cell system

A technology of pancreatic duct and stem cell line, applied in the field of animal cell (tissue) engineering, can solve the problem that there is no report on rat pancreatic duct epithelial-like stem cell line.

Inactive Publication Date: 2014-06-25
GUANGDONG OCEAN UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the rat pancreatic ductal epithelial-like stem cell line in China

Method used

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  • Rat pancreatic duct epithelioid stem cell system
  • Rat pancreatic duct epithelioid stem cell system
  • Rat pancreatic duct epithelioid stem cell system

Examples

Experimental program
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Embodiment 1

[0045] 1. Morphological characteristics of rat pancreatic ductal epithelial-like stem cells

[0046] H·E staining: rat pancreatic ductal epithelioid stem cells showed polygonal epithelioid after complete attachment ( figure 1 ). The staining method refers to Situ Zhenqiang, Wu Junzheng. Cell Culture [M]. World Book Publishing Company. 2007. Slightly;

[0047] Giemsa staining: rat pancreatic ductal epithelioid stem cells have a round or oval nucleus with 1 to 3 nucleoli ( figure 2 ). The staining method refers to R.I. Frescheini. Animal cell culture-basic technical guide [M]. Science Press. 2008. Slightly;

[0048] 2. Growth characteristics of rat pancreatic ductal epithelial-like stem cells

[0049] The clonal growth of pancreatic ductal epithelial stem cells in rats; the growth curve showed that the cells grew slowly on the 1st to 2nd day after inoculation, grew logarithmically on the 3rd to 6th day, and the proliferation ability decreased on the 7th day ( image 3 ). ...

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Abstract

The invention relates to a rat pancreatic duct epithelioid stem cell system, and belongs to the field of animal cell (tissue) engineering. The rat pancreatic duct epithelioid stem cell is from a pancreatic duct tissue of a normal adult rat, can achieve clonal growth if being cultured in vitro, and has multi-lineage differentiation potential, specifically, the cell adherent culture is polygonal epithelioid, a cell nucleus is circular or elliptic, and 1-3 nucleoluses grow in the cell nucleus; cells achieve clonal growth, the cells grow slowly after being inoculated in the first to second days and achieve logarithmic growth in the 3-6th days, and the multiplication capacity begins to reduce in the 7th day; the cells are normal diploid cells, and the chromosome number is 2n=42; a proliferating cell nuclear antigen, an octamer transcription factor 4, stem cell key protein, keratin 19 and a neurogenic differentiation factor 2 are expressed on a protein expression level; the rat pancreatic duct epithelioid stem cell system has multi-lineage differentiation potential and is differentiated into neural cells, adipose cells and osteoblasts by virtue of directional induction in vitro; and the rat pancreatic duct epithelioid stem cell system has no tumorigenicity.

Description

technical field [0001] The present invention relates to human and other mammalian pancreatic ductal stem cell lines and their characteristics, in particular to a rat pancreatic ductal epithelial stem cell line and its biological characteristics such as morphology, growth, expression and differentiation, and belongs to the field of animal cell (tissue) engineering. Background technique [0002] Diabetes mellitus (DM) is one of the major diseases that endanger human health. According to statistics, the number of diabetic patients in the world has reached 346 million (Am J Stem Cells.2012,1(3):196-204.), while the traditional Drugs and insulin replacement therapy can not fundamentally cure the disease. In the past ten years, with the development of transplantation technology, pancreatic islet transplantation has achieved certain curative effect in the treatment of diabetes (N Engl J Med.2000,343(4):230-238. Diabetes.2005,54(7):2060-2069. Am J Transplant. 2008,8(11):2463-2470. ...

Claims

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Application Information

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IPC IPC(8): C12N5/071
Inventor效梅安立龙
OwnerGUANGDONG OCEAN UNIVERSITY