Breeding method for disease-resistant functional rice two-line sterile line
A functional, sterile line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve problems such as low water-soluble protein content, achieve good nutritional and health care effects, excellent agronomic and yield traits, Enhances gut health effects
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Embodiment 1
[0021] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0022] 3) Plant F 1-ABCD F 2-ABCD , planting F 2-ABCD , with a population of 2,800 plants, identify rice blast resistance at the seedling stage, select and mark plants with high blast resistance, harvest seeds from individual plants at the mature stage, measure resistant starch content, water-soluble protein content, and vitamin A content, and obtain by screening F which is high in resistant starch, vitamin A and low in water soluble protein 3-ABCD seed;
[0023] 4) 20 copies of strain F 3-ABCD , conduct identification of rice blast induced in the field, select excellent individual plants of strains with high resistance to rice blast and excellent agronomic and yield traits for harvesting, measure resistant starch content, water-soluble protein content, and vitamin A content, and screen to obtain resistance F with high starch content,...
Embodiment 2
[0032] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0033] 3) Plant F 1-ABCD F 2-ABCD , planting F 2-ABCD , with a population of 3,000 plants, identifying rice blast resistance at the seedling stage, selecting and marking plants with high blast resistance, harvesting seeds from individual plants at the mature stage, measuring the content of resistant starch, water-soluble protein, and vitamin A, and screening to obtain F which is high in resistant starch, vitamin A and low in water soluble protein 3-ABCD seed;
[0034] 4) 20 copies of strain F 3-ABCD , conduct identification of rice blast induced in the field, select excellent individual plants of strains with high resistance to rice blast and excellent agronomic and yield traits for harvesting, measure resistant starch content, water-soluble protein content, and vitamin A content, and screen to obtain resistance F with high starch con...
Embodiment 3
[0043] 2) Planting hybrid F 1 generation, crossed F at flowering stage 1-AB / F 1-CD , have to resubmit F 1 , with F 1-ABCD express;
[0044] 3) Plant F 1-ABCD F 2-ABCD , planting F 2-ABCD , with a population of 3,000 plants, identifying rice blast resistance at the seedling stage, selecting and marking plants with high blast resistance, harvesting seeds from individual plants at the mature stage, measuring the content of resistant starch, water-soluble protein, and vitamin A, and screening to obtain F which is high in resistant starch, vitamin A and low in water soluble protein 3-ABCD seed;
[0045] 4) 20 copies of strain F 3-ABCD , conduct identification of rice blast induced in the field, select excellent individual plants of strains with high resistance to rice blast and excellent agronomic and yield traits for harvesting, measure resistant starch content, water-soluble protein content, and vitamin A content, and screen to obtain resistance F with high starch con...
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