A kind of highly active bio-derived material and its preparation method and application
A highly active and biological technology, applied in the field of medical skin tissue engineering, can solve the problems of reducing the content of biologically active factors in derivative materials and complex preparation processes, achieving high safety and effectiveness, simplified preparation processes, and low antigenicity
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Embodiment 1
[0032] The bio-derived material described in this example is prepared according to the following steps:
[0033] (1) Wash the sheep small intestine with tap water, then treat the sheep small intestine with a mixed aqueous solution of 0.1% peracetic acid and 17% ethanol, and then rinse it with purified water;
[0034] (2) Manually scrape off the muscular layer, serosal layer and mucosal layer of the above-mentioned cleaned and disinfected sheep small intestine to obtain 4 kg of the submucosa, and put the submucosa into a temperature-controlled ultrasonic cleaning system with automatic water intake and drainage functions. In the cleaning tank of the machine, alternately use hypertonic pressure saline solution and purified water to carry out vibration immersion treatment on the submucosa under ultrasonic conditions. The ultrasonic immersion steps are as follows: 25L of 4% hypertonic chlorine The sodium chloride solution is discharged into the cleaning tank of the temperature-cont...
Embodiment 2
[0037] The bio-derived material described in this example is prepared according to the following steps:
[0038] (1) Take the pig small intestine and wash it with tap water, then treat the submucosa of the pig small intestine with a mixed aqueous solution of 3% peracetic acid and 15% ethanol, and then rinse it with purified water;
[0039] (2) Manually scrape off the pig small intestinal submucosa after cleaning and disinfection to obtain 5 kg of the small intestinal submucosa, put the submucosa into the cleaning pool of a temperature-controlled ultrasonic cleaning machine with automatic water inlet and drainage functions, alternately Use hyperosmotic sodium chloride solution and purified water to carry out vibration soaking treatment on the submucosa under ultrasonic conditions, and the ultrasonic soaking steps are as follows: discharge 10 L of hyperosmotic sodium chloride solution with a mass concentration of 20% into In the cleaning tank of a temperature-controlled ultrason...
Embodiment 3
[0042] The bio-derived material described in this example is prepared according to the following steps:
[0043] (1) Take the pig small intestine and wash it with tap water, then treat the pig small intestine with a mixed aqueous solution of 3% peroxypropionic acid and 15% isopropanol, and then rinse it with purified water;
[0044] (2) Manually scrape off the pig small intestinal submucosa after cleaning and disinfection to obtain 0.5 kg of the small intestinal submucosa, and put the small intestinal submucosa into the cleaning pool of a temperature-controlled ultrasonic cleaning machine with automatic water inlet and drainage functions , alternately using hyperosmotic pressure sodium chloride solution and purified water to carry out vibration soaking treatment on the submucosa under ultrasonic conditions, the ultrasonic soaking steps are as follows: 1.5 L of 30% hyperosmotic sodium chloride solution Discharge into the cleaning tank of a temperature-controlled ultrasonic clea...
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