A kind of highly active bio-derived material and its preparation method and application

A highly active and biological technology, applied in the field of medical skin tissue engineering, can solve the problems of reducing the content of biologically active factors in derivative materials and complex preparation processes, achieving high safety and effectiveness, simplified preparation processes, and low antigenicity

Inactive Publication Date: 2016-08-31
苏州期佰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] For this reason, the technical problem to be solved by the present invention is that the method for preparing biologically derived materials in the prior art is in order to completely remove the immune activity of antigens, which leads to the problem that the preparation process is complicated while significantly reducing the content of biologically active factors in the derived materials, and then provides a preparation of high facile process for active bio-derived materials;

Method used

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  • A kind of highly active bio-derived material and its preparation method and application
  • A kind of highly active bio-derived material and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The bio-derived material described in this example is prepared according to the following steps:

[0033] (1) Wash the sheep small intestine with tap water, then treat the sheep small intestine with a mixed aqueous solution of 0.1% peracetic acid and 17% ethanol, and then rinse it with purified water;

[0034] (2) Manually scrape off the muscular layer, serosal layer and mucosal layer of the above-mentioned cleaned and disinfected sheep small intestine to obtain 4 kg of the submucosa, and put the submucosa into a temperature-controlled ultrasonic cleaning system with automatic water intake and drainage functions. In the cleaning tank of the machine, alternately use hypertonic pressure saline solution and purified water to carry out vibration immersion treatment on the submucosa under ultrasonic conditions. The ultrasonic immersion steps are as follows: 25L of 4% hypertonic chlorine The sodium chloride solution is discharged into the cleaning tank of the temperature-cont...

Embodiment 2

[0037] The bio-derived material described in this example is prepared according to the following steps:

[0038] (1) Take the pig small intestine and wash it with tap water, then treat the submucosa of the pig small intestine with a mixed aqueous solution of 3% peracetic acid and 15% ethanol, and then rinse it with purified water;

[0039] (2) Manually scrape off the pig small intestinal submucosa after cleaning and disinfection to obtain 5 kg of the small intestinal submucosa, put the submucosa into the cleaning pool of a temperature-controlled ultrasonic cleaning machine with automatic water inlet and drainage functions, alternately Use hyperosmotic sodium chloride solution and purified water to carry out vibration soaking treatment on the submucosa under ultrasonic conditions, and the ultrasonic soaking steps are as follows: discharge 10 L of hyperosmotic sodium chloride solution with a mass concentration of 20% into In the cleaning tank of a temperature-controlled ultrason...

Embodiment 3

[0042] The bio-derived material described in this example is prepared according to the following steps:

[0043] (1) Take the pig small intestine and wash it with tap water, then treat the pig small intestine with a mixed aqueous solution of 3% peroxypropionic acid and 15% isopropanol, and then rinse it with purified water;

[0044] (2) Manually scrape off the pig small intestinal submucosa after cleaning and disinfection to obtain 0.5 kg of the small intestinal submucosa, and put the small intestinal submucosa into the cleaning pool of a temperature-controlled ultrasonic cleaning machine with automatic water inlet and drainage functions , alternately using hyperosmotic pressure sodium chloride solution and purified water to carry out vibration soaking treatment on the submucosa under ultrasonic conditions, the ultrasonic soaking steps are as follows: 1.5 L of 30% hyperosmotic sodium chloride solution Discharge into the cleaning tank of a temperature-controlled ultrasonic clea...

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Abstract

The invention discloses a method for preparing a high-activity bio-derived material. The method comprises the following steps: (1) taking the submucosa materials of animals as raw materials, and cleaning and disinfecting for the future use; (2) placing the materials subjected to the disinfection treatment in hyperoncotic sodium chloride solution, oscillating and soaking for 1-20 minutes in an ultrasonic condition, taking the treated materials and adding purified water to soak, oscillating and soaking for 1-20 minutes in the ultrasonic condition, then draining the added water, and repeatedly and cyclically operating for 4-6 times respectively; (3) carrying out descaling and cleaning treatments on the materials subjected to the soaking treatment in the step (2), and freeze-drying to obtain the high-activity bio-derived material. According to the method disclosed by the invention, the problems of long treatment time and remarkable bioactivity lowering due to that cellular antigens cannot be effectively removed or the process steps are complex and various, of a pure mechanical method in the prior art are solved.

Description

technical field [0001] The invention belongs to the technical field of medical skin tissue engineering, and in particular relates to a biologically derived material with high biological activity and a preparation method thereof. Background technique [0002] Biologically derived materials have a structure similar to extracellular matrix and good biocompatibility, and are a very advantageous scaffold material for tissue engineering. Among many bio-derived materials, small intestinal submucosa (SIS) has the advantages of low immunogenicity, antibacterial properties and tissue growth promotion, so it is considered to be an ideal scaffold material for tissue engineering. [0003] SIS is rich in proteoglycan, collagen and fibronectin, which can promote the adhesion, proliferation and differentiation between cells and tissues. Proteoglycans often play a role in the early stages of cell adhesion, such as cell attachment and stretching stages; collagen passes through the α1 chain o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61L27/36A61L27/54
Inventor 韩立军
Owner 苏州期佰生物技术有限公司
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