Process of preparing Physalis tofu by fermenting yellow pulp water with lactic acid bacteria
A technology of lactic acid bacteria fermentation and yellow pulp water, applied in dairy products, cheese substitutes, applications, etc., can solve the problems of non-standardization, miscellaneous bacteria-contaminated sour pulp production and use, etc., to reduce the use of water, reduce production costs, The effect of increasing acidity
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Embodiment 1
[0034] 1) Strain activation: take Lactobacillus plantarum FM-LP1-1 strain, inoculate in 30mL of yellow pulp water, activate and cultivate at 37°C for 20 hours; seed expansion in triangular flask: inoculate to 1L according to the inoculum size 3% (v / v) In yellow pulp water, expand culture at 37°C for 8 hours until the number of viable cells reaches 10 8 More than CFU / mL; plant-level seed preparation: inoculate 30L of yellow pulp water at 3% (v / v) inoculum size, expand culture at 37°C for 8 hours until the number of viable cells reaches 10 8More than CFU / mL; Physalis fermentation: Inoculate the primary seed liquid into 1t of yellow pulp water at an inoculum volume of 3% (v / v), ferment at 37°C for 20 hours until the final pH value reaches 4.10.
[0035] 2) When the pH value at the end point of Physalis fermentation is 4.10, correspondingly add 0.65% food-grade glacial acetic acid to adjust the pH to 3.90 to obtain a tofu coagulant.
[0036] 3) Select commercially available small...
Embodiment 2
[0038] 1) Strain activation: take Lactobacillus plantarum FM-LP1-1 strain, inoculate in 30mL of yellow pulp water, activate and cultivate at 37°C for 20h; seed expansion in triangular flask: inoculate to 1L according to the inoculum size 3% (v / v) In yellow pulp water, expand culture at 37°C for 8 hours until the number of viable cells reaches 10 8 More than CFU / mL; plant-level seed preparation: inoculate 30L of yellow pulp water with 3% (v / v) inoculum amount, expand culture at 37°C for 8 hours until the number of viable cells reaches 10 8 More than CFU / mL; Second-level seed preparation in the workshop: inoculate 3% (v / v) of the inoculum into 1t of yellow pulp water, expand the culture at 37°C for 8h until the number of viable cells reaches 10 8 More than CFU / mL; physalis fermentation: Inoculate the secondary seed liquid into 30t of yellow pulp water according to the inoculation amount of 3% (v / v), ferment at 37°C for 20h until the final pH value reaches 4.05
[0039] 2) When ...
Embodiment 3
[0042] 1) Strain activation: take Lactobacillus plantarum FM-LP1-1 strain, inoculate in 30mL of yellow pulp water, activate and cultivate at 37°C for 20h; seed expansion in triangular flask: inoculate to 1L according to the inoculum size 3% (v / v) In yellow pulp water, expand culture at 37°C for 8 hours until the number of viable cells reaches 10 8 More than CFU / mL; Physalis fermentation: Inoculate the seed liquid into 30L yellow pulp water according to the inoculum amount 3% (v / v), ferment at 37°C for 20h until the final pH value reaches 4.20.
[0043] 2) When the pH value at the end point of Physalis fermentation is 4.20, correspondingly add 0.82% food-grade glacial acetic acid to adjust the pH to 3.90 to obtain a tofu coagulant.
[0044] 3) Remove impurities and wash soybeans, soak beans with soybean water mass ratio 1:3 (tap water), soak for 10-12 hours (spring), so that soybeans are fully soaked and ready for use. Unsoaked soybeans are removed during use, and the quality ...
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