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A detection kit for auxiliary diagnosis of Alzheimer's disease and its detection method

A technology for Alzheimer's disease and detection kits, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of no public detection kits, etc., and achieve the effect of strong pertinence and high detection efficiency

Inactive Publication Date: 2015-09-09
NINGBO UNIV
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Problems solved by technology

[0004] At present, there are no research reports on the detection kits used to detect the degree of methylation in the promoter region of the brain-derived neurotrophic factor gene associated with Alzheimer's disease at home and abroad.

Method used

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  • A detection kit for auxiliary diagnosis of Alzheimer's disease and its detection method
  • A detection kit for auxiliary diagnosis of Alzheimer's disease and its detection method

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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0029] A detection kit for auxiliary diagnosis of Alzheimer's disease, the detection kit includes a pair of brain-derived neurotrophic factor gene promoter region methylation-specific amplification primers and a methylation-specific sequencing primer , the pair of brain-derived neurotrophic factor gene promoter region methylation-specific amplification primers include methylation-specific upstream primers and methylation-specific downstream primers:

[0030] The methylation-specific upstream primers include the following nucleotide sequences:

[0031] 5'-TTAGTATTTAAGAGGAAAAGGGAAAGTTGT-3';

[0032] The methylation-specific downstream primers include the following nucleotide sequences:

[0033] 5'-Biotin-CCCCCATCATAACTAAAAATCT-3';

[0034] The methylation-specific sequencing primers include the following nucleotide sequences:

[0035] 5'-GGG...

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Abstract

The invention discloses a detection kit for helping diagnose Alzheimer disease. The detection kit comprises a pair of brain derived neuro-trophic factor gene promoter region methylated specific amplification primers and methylated specific sequencing primer, wherein the pair of brain derived neuro-trophic factor gene promoter region methylated specific amplification primers consist of a methylated specific forward primer and a methylated specific reverse primer; detecting the methylation degree of a brain derived neuro-trophic factor gene promoter region can help diagnose Alzheimer disease, the detection kit is simple and convenient, high in detection efficiency, strong in pertinence, has the advantages of being accurate and reliable, flexible, fast and economical, thus being beneficial to early discovery and timely treatment of the Alzheimer disease.

Description

technical field [0001] The present invention relates to the technical field of auxiliary diagnosis of Alzheimer's disease, in particular to a detection kit for auxiliary diagnosis of Alzheimer's disease and a detection method thereof, in particular to a detection kit that can be used to detect Alzheimer's disease-related A kit for detecting the degree of methylation in the promoter region of the brain-derived neurotrophic factor gene and a detection method thereof. Background technique [0002] Alzheimer's disease (Alzheimer disease, AD) is a systemic neurodegenerative disease, which is clinically manifested as high-level cognitive decline, and its main features include intracellular neurofibrillary tangles and extracellular senile plaques. Patients with Alzheimer's disease require life-long care, thus placing a great financial burden on society and families. Alzheimer's disease is a complex disease caused by the joint action of genetic factors and environmental factors. Fi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q1/6883C12Q2600/154C12Q2531/113C12Q2565/301C12Q2523/125
Inventor 季慧慧段世伟王钦文徐旭婷
Owner NINGBO UNIV
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