Boar semen refrigerating method and dedicated refrigerating fluid thereof

Abstract

The invention discloses a boar semen refrigerating method and dedicated refrigerating fluid thereof. The method comprises the following steps: (1) diluting dense boar semen according to a volume ratio of 1:(0.5-3), stewing at 26-30 DEG C for 25-40 minutes, and centrifuging 800g of boar semen for 10 minutes to obtain sperm precipitation; (2) adding refrigerating fluid I into the sperm precipitation according to a volume ratio of 1: (1-1.5), stewing at 17-20 DEG C for 1-2 hours, and stewing at 4-7 DEG C for 2 hours to obtain a system I; (3) adding refrigerating fluid II containing glycerin into the system I to obtain a system II with final glycerin concentration of 3%; (4) stewing the system II at 4 DEG C for 1-2 hours, and adding caffeine to obtain a system III with final caffeine concentration of 0.2g / L; and (5) stewing the system III at 4 DEG C for 0.5-2 hours, dropwise refrigerating at -120 DEG C, stewing and balancing for 5-10 minutes, and submerging the system III in liquid nitrogen for cryopreservation. After the boar semen refrigerated by the method is thawed, the sperm survival rate of the boar semen is higher than 0.45, and the in vitro survival time of the boar semen is longer than 6 hours, thereby reaching the production application level. The boar semen refrigerating method disclosed by the invention is used for providing scientific basis for the application and popularization of the refrigerated boar semen in production, and providing a technical method and standard for boar semen refrigerating.

Description

technical field [0001] The invention relates to a pig semen freezing method and a special freezing liquid thereof. Background technique [0002] With the popularization of pig artificial insemination technology, semen cryopreservation technology has an irreplaceable role in preserving high-quality germplasm resources, can prolong the service life of breeding pigs, and is not limited by factors such as the death, injury, and reproductive diseases of the boar; it can effectively Give full play to the role of high-quality boars and improve the utilization rate of high-quality breeding pigs; the introduction of high-quality boar semen can replace the introduction of live boars, reducing the cost of breeding. However, in the process of freezing pig semen, many internal and external factors will greatly affect the freezing effect. The internal factors mainly include: the internal characteristics of pig sperm, the differences between different pigs, and even different ejaculatory p...

AI Technical Summary

Problems solved by technology

Polyunsaturated fatty acids are prone to lipid peroxidation under the action of reactive oxygen species (ROS), which reduces the number of polyunsaturated fatty acids in the plasma membrane. Since the plasma membrane of pig sperm contains a large amount of polyunsaturated fatty acids, it is extremely sensitive to oxidative attacks, which eventually leads to Sperm motility and fertilization ability are reduced, making artificial insemination with frozen semen much less effective than fresh semen insemination and natural mating

Despite decades of unremitting efforts, the conception rate and litter size of frozen semen are still far lower than those of normal temperature liquid semen