Compound degreasing liquid for extracting chicken egg yolk immunoglobulin and its application
A technology of immunoglobulin and chicken egg yolk, which is applied in the field of protein separation and purification, can solve the problems of protein variability, complicated operation, and low yield, and achieve the effects of maintaining biological activity, good segmentation effect, and low cost
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Embodiment 1
[0039] Prepare a compound fat-removing liquid for chicken yolk immunoglobulin extraction, in which carrageenan is selected as the polysaccharide, and a group of compound fat-removing liquids containing different final concentrations of carrageenan is prepared, numbered as group A1, and a group is prepared with different final concentrations The composite degreasing solution of pectin, number A2 group, prepare a group of composite degreasing solutions containing ammonium sulfate with different final concentrations, number A3 group, the above-mentioned composite degreasing solution is an aqueous solution, and each component contained in it And the weight percentage content is shown in the table below:
[0040] Group A1 pectin carrageenan ammonium sulfate A11 0.1% 0.01% 3% A12 0.1% 0.05% 3% A13 0.1% 0.1% 3% A14 0.1% 0.15% 3% A15 0.1% 0.2% 3% Group A2 pectin carrageenan ammonium sulfate A21 0.01% 0.1% 3% A22 ...
Embodiment 2
[0043] Prepare a compound degreasing solution for chicken yolk immunoglobulin extraction, in which carboxymethyl cellulose is selected as the polysaccharide, and a group of compound degreasing solutions containing carboxymethyl cellulose with different final concentrations are prepared, group number B1, prepared A group of composite degreasing solutions containing pectin with different final concentrations, coded as group B2, prepared a group of composite degreasing solutions containing ammonium sulfate with different final concentrations, coded as group B3, the above-mentioned composite degreasing solutions are aqueous solutions, The components and weight percentages contained in it are shown in the following table:
[0044] Group B1 pectin carboxymethyl cellulose ammonium sulfate B11 0.1% 0.01% 3% B12 0.1% 0.05% 3% B13 0.1% 0.1% 3% B14 0.1% 0.15% 3% B15 0.1% 0.2% 3% Group B2 pectin carboxymethyl cellulose ammonium ...
Embodiment 3
[0047] Prepare a compound fat-removing solution for chicken yolk immunoglobulin extraction, in which methylcellulose is selected as the polysaccharide, and a group of compound-type fat-removing solutions containing methylcellulose with different final concentrations are prepared, numbered as group C1, and a group is prepared Composite degreasing solution containing pectin with different final concentrations, coded as group C2, preparing a group of composite degreasing solutions containing ammonium sulfate with different final concentrations, coded as group C3, the above-mentioned composite degreasing solution is an aqueous solution, which contains Each component and weight percentage content of each composition are shown in the table below:
[0048] Group C1 pectin Methylcellulose ammonium sulfate C11 0.1% 0.01% 3% C12 0.1% 0.05% 3% C13 0.1% 0.1% 3% C14 0.1% 0.15% 3% C15 0.1% 0.2% 3% Group C2 pectin Methylcellulose amm...
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