Biotransformation method for generating rebaudioside E (RE) with stevioside (ST)

Abstract

The invention discloses a biotransformation method for generating rebaudioside E (RE) with stevioside (ST). The biotransformation method is characterized by transforming ST into RE by adopting aspergillus niger II under the action of an additional sugar source. The biotransformation method has the advantages that the transformation rate of RE obtained by using ST as the raw material is not less than 70%; the content of RE in solid matters can be at least 60% and can be 80% and even more than 90% after further separation and purification; RE has taste similar to that of sucrose and can be independently used as a sweetening agent; the method is low in RE production cost, is easy to expand and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of food chemical industry, and in particular relates to a biotransformation method for producing rebaudioside E from stevioside. Background technique [0002] Stevioside (Steviolglycosides) is a general term for stevia extract, its sweet components include stevioside (Stevioside), rebaudioside A, B, C, D, E (RebaudiosideA / B / C / D / E) , steviolbioside (Steviolbioside), dulcoside A (DulcosideA) and so on. In traditional steviol glycosides, stevioside accounts for about 66% of the total glycosides, rebaudioside A accounts for about 22% of the total glycosides, rebaudioside C accounts for about 9% of the total glycosides, and dulcoside A accounts for about 2% of the total glycosides, and very little content of other ingredients. The structural formulas of the main components of steviol glycosides are shown below. [0003] [0004] Stevioside (Stevioside, hereinafter referred to as ST) is the sweet component with the mo...

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Problems solved by technology

[0011] So far, there has been no report on the production of rebaudioside E (Rebaudioside E) by biotransformation with sucrose as a gl

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