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Method for detecting fungal flora during Liupu tea processing process

A processing and detection method technology, applied in the field of molecular biology, can solve the problem of whether the detection and analysis method is still blank

Inactive Publication Date: 2014-07-23
GUANGXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection and analysis methods for the microbial community structure of Liubao tea are still blank

Method used

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  • Method for detecting fungal flora during Liupu tea processing process
  • Method for detecting fungal flora during Liupu tea processing process
  • Method for detecting fungal flora during Liupu tea processing process

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Take samples of Liubao tea in different fermentation processes

[0035] Extract the tea leaves from the 266th batch of fermentation workshop in Wuzhou Tea Factory in 2012. The weight of the tea pile is about 20 tons. The height of the tea pile is about 60cm, and the tea leaves from the upper layer (including the pile surface and 5cm below the surface), the middle layer (about 20-30cm away from the pile surface), and the lower layer (including the pile bottom and 5cm above the ground) are extracted. The four corners and the middle points of the upper, middle and lower layers are respectively taken, and a total of 5 points are taken, and then they are mixed in a sterile room to make a tea mixture sample containing the upper, middle and lower layer tea leaves. Extract the large basket of tea leaves that have just entered the aging period from the aging warehouse, each about 50kg, split them with a knife to take the middle part, take 2 pieces in total, and mix them evenly ...

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Abstract

The invention discloses a method for detecting fungal flora during a Liupu tea processing process, which is characterized in that fungi total DNA is extracted from Liupu tea during the processing process, and then a PCR-DGGE technology is employed for detecting the fungal flora composition. According to the invention, defect that the uncultured fungi microbe in Liupu tea can not be separated and determined by a traditional culture can be avoided, the experiment result can be obtained in a short time, workload and work time can be greatly reduced, and the experiment result is true and can be trusted. The method is suitable for the Liupu tea and a tea sample during the Liupu tea processing process, the detected fungal flora comprehensively comprises fungi types in the Liupu tea, fungal flora dynamic change of Liupu tea during the processing process can be objectively and effectively reflected, and the method establishes a scientific base for effectively revealing the effect mechanism of the microbe during a Liupu tea fermentation process and for improving a Liupu tea production technology.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for detecting fungal flora during Liubao tea processing. Background technique [0002] There are a large number of microorganisms in the production process of dark tea, and the types of microorganisms in different dark tea processing are different. It can be said that it is a rather complicated micro-ecosystem with tea as the substrate. In this system, bacteria, fungi, and actinomycetes are all interdependent or antagonistic to each other, and as the fermentation progresses, various types of microorganisms are also undergoing great changes, during which a large number of contained substances are produced, such as soluble polysaccharides, polysaccharides, etc. phenol, protease, etc. Therefore, it is of great significance to study the microbial community structure and diversity in the micro-ecosystem in black tea processing for the formation, quality...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/686C12Q2565/125
Inventor 卢洁温志杰施向东莫祺红李小娟吴志勇吴平何志强白先放何勇强
Owner GUANGXI UNIV
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