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A kind of method of raising Lepidoptera insect

A technology for Lepidoptera insects and adults, applied in chemistry, animal husbandry, etc., can solve the problems of egg hatching rate, larva pupation rate, pupa emergence rate and adult survival rate, labor-intensive capture of adults, and adult worms waiting for opportunities to escape. Achieve the effects of increasing egg hatching rate and pupae emergence rate, inhibiting bacterial proliferation, and preventing insects from escaping

Inactive Publication Date: 2015-08-19
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned wooden cages or breeding boxes need to open the cage door frequently when changing food or collecting insect eggs during the breeding process. Not only is the operation cumbersome, but it is also easy to cause the adults to escape. It is often time-consuming and labor-intensive to catch the escaped adults in the laboratory. , and the feeding efficiency is relatively low
However, traditional rearing methods are difficult to control the degree of disinfection in the disinfection process of raising wooden cages or breeding boxes, often resulting in incomplete disinfection, resulting in a large number of bacteria and viruses, so that larvae, pupae, etc. are infected with bacteria and viruses. Therefore, the above disinfection process Defects often lead to low egg hatching rate, larval pupation rate, pupal emergence rate and adult survival rate, which is not conducive to experimental research and practical application.

Method used

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  • A kind of method of raising Lepidoptera insect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Feeding armyworms:

[0034](1) Egg collection and disinfection: 2-3 days after adult eclosion in tray-type cages, plastic ropes were hung on the top of the cage, and eggs were collected from plastic ropes on the 5th day after adult eclosion, and then collected once a day for a total of Collect for 8-9 days; after the eggs are collected, sterilize the plastic rope with the eggs with a formaldehyde solution with a volume concentration of 3.7% for 15 minutes, then take them out with sterile tweezers and soak them in sterile water for 3 times, each time for 30 minutes; the soaking is over Then take it out and put it indoors to dry naturally;

[0035] (2) Egg hatching larvae and larva rearing: Spread 2-3 layers of absorbent paper on the bottom of the sterile feeding bottle, then put the plastic rope dried in step 1 in the sterile feeding bottle, seal it and place it in the light for cultivation Cultivate in an incubator under the conditions of temperature 22°C and photoperi...

Embodiment 2

[0041] Feeding of beet armyworm:

[0042] (1) Egg collection and disinfection: On the 2nd day after the adults in the tray-type cages eclosion, hang the plastic rope on the top of the cage, and collect the eggs from the plastic rope on the 3rd day after the adult eclosion, and then collect once a day for a total of 3 -5d; After the eggs are collected, sterilize the plastic rope with the eggs with a formaldehyde solution with a volume concentration of 3.7% for 10 minutes, then take them out with sterile tweezers and soak them in sterile water for 3 times, each time for 20 minutes; take them out after soaking Put it indoors to dry naturally;

[0043] (2) Egg hatching larvae and larva rearing: Spread 2-3 layers of absorbent paper on the bottom of the sterile feeding bottle, then put the plastic rope dried in step 1 in the sterile feeding bottle, seal it and place it in the light for cultivation Cultivate in an incubator under the conditions of temperature 28°C and photoperiod L:...

Embodiment 3

[0049] Feeding of cotton bollworm:

[0050] (1) Egg collection and disinfection: 2-3 days after the adults in the tray-type cages emerged, wrap gauze around the cages, collect eggs from the gauze on the 4th day after the adults emerged, and collect them once a day thereafter. 5-8d; After the eggs are collected, sterilize the gauze with the eggs with a formaldehyde solution with a volume concentration of 3.7% for 10 minutes, then take them out with sterile tweezers and soak them in sterile water for 3 times, each time for 20 minutes; take them out after soaking Put it indoors to dry naturally;

[0051] (2) Egg hatching larvae and larva rearing: Spread 2-3 layers of absorbent paper on the bottom of the sterile feeding bottle, then put the gauze dried in step 1 in the sterile feeding bottle, seal it and place it in the light incubator Medium culture, the culture conditions are temperature 25°C, photoperiod L:D=14h:10h, after 3-4 days of culture, the eggs hatch into larvae; after...

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Abstract

The invention discloses a method for feeding lepidopteron. In the feeding process, a tray type net cage is used as a main feeding tool. The method comprises steps of collecting eggs, sterilizing, hatching larvae from the eggs, feeding the larvae, feeding pupas, feeding imagoes and the like, wherein gauze or plastic ropes are used as an oviposition substrate for collecting the eggs; the eggs are sterilized by using a formaldehyde solution with the volume concentration of 3-4%; the eggs are hatched and fed in an illumination incubator; the larvae are sterilized by using a sodium hypochlorite solution with the volume concentration of 8-12%; pupas feather in the tray type net cage; the feathering imagoes are continuously fed in the net cage and the eggs are collected and sterilized. The tray type net cage is simple and convenient to operate, the insects are effectively prevented from escaping and the cleaning can be carried out at any time; in the feeding process, eggs and larvae are disinfected by using different disinfectants, the disinfection effect is thorough and gentle, the bacteria are effectively prevented from mass propagation, and the hatchability of the eggs, the feathering rate of pupa are also improved, the hatchability of the eggs can be 95-96%, and the feathering rate of pupa can be 98%.

Description

technical field [0001] The invention relates to a test method for lepidopteran adult eclosion, egg collection, larva and pupa feeding, in particular to a method for raising lepidopteran insects. Background technique [0002] Lepidoptera is the second largest order of Insecta. Lepidoptera insects such as silkworm, tussah silkworm and castor silkworm are very important economic insects. Cordyceps sinensis, which is formed by the parasitization of bat moth larvae by fungi, is even more valuable Chinese herbal medicine; , cotton bollworm, armyworm, beet armyworm, etc. are important agricultural pests, causing huge economic losses every year. [0003] Lepidoptera insects need to go through four stages of eggs, larvae, pupae and adults to complete a generation. Larvae are usually raised with artificial feed, and the technology is relatively mature; adult insects must provide a large feeding space so that the male and female can mate before they can lay eggs and reproduce offspring...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A61L2/18
Inventor 周洪旭谭秀梅孙冬姚秀锋
Owner QINGDAO AGRI UNIV
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