Culture medium for promoting differentiation of rat neural stem cells and use method of culture medium

Abstract

The invention discloses a culture medium for promoting differentiation of rat neural stem cells and a use method of the culture medium. The culture medium contains B-27 Supplement without retinoic acid, L-Glutamine, FBS (fetal bovine serum), beta-NGF (neural growth factor), BDNF (brain derived neurotrophic factor), RA and vitamin E in Neurobasal-A medium. The culture medium is low in cost, the problem of low neural stem cell differentiation efficiency in the prior art is solved, and the efficiency of differentiating neural stem cells into nerve cells is remarkably improved.

Description

technical field

[0001] The invention belongs to the technical field of neural stem cell culture and differentiation, and in particular relates to a culture medium for promoting the differentiation of rat neural stem cells and a use method thereof. Background technique

[0002] Traditional views generally believe that the self-repair ability of the central nervous system is limited, and neurons after injury will not be able to regenerate. Nevertheless, scholars all over the world are trying to challenge this traditional concept. Since Reynolds and Weiss isolated and cultured neural stem cells from striatal tissue of mice in the 1990s, people have not only isolated and cultured neural stem cells from embryos of various animals and humans, but also from central nervous system tissues of adult animals and humans. Neural stem cells have been cultured and proved to have strong proliferation and self-renewal capabilities, breaking this traditional concept. The academic circles cu...

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