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The use of mir‑218‑5p compounds as markers of chronic pain and drugs for the treatment of inflammatory chronic pain

A chronic arthritis and drug technology, applied in the field of biomedical materials, can solve problems that have not been reported, and achieve the effects of simple process, lower heat shock threshold and low cost

Inactive Publication Date: 2017-06-20
XUZHOU MEDICAL COLLEGE
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Problems solved by technology

[0005] The application of miR-218-5p as a clinical diagnostic marker for chronic pain diseases and in the preparation of drugs for the treatment of chronic pain has not been reported yet

Method used

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  • The use of mir‑218‑5p compounds as markers of chronic pain and drugs for the treatment of inflammatory chronic pain
  • The use of mir‑218‑5p compounds as markers of chronic pain and drugs for the treatment of inflammatory chronic pain
  • The use of mir‑218‑5p compounds as markers of chronic pain and drugs for the treatment of inflammatory chronic pain

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Embodiment Construction

[0024] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0025] 1. Preparation of CFA chronic arthritis pain model and expression of miR-218-5p at spinal cord level:

[0026] SPF-grade male Kunming male mice of 6 weeks were anesthetized with isoflurane, and injected 40 μl of sterile complete Freund's adjuvant (CFA; Sigma Company) intradermally at two points around the metatarsal joint of the left hind foot. Injection concentrations can induce arthritis. The decrease in thermal and ...

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Abstract

The invention relates to an application of miRNA in preparation of a medicine for treating inflammation type chronic pain, and in particular relates to an application of a miR-218-5p compound. The application comprises the steps: (1) screening a miR-218-5p target gene, verifying the gene through a reporter gene, and culturing an inflammation type chronic pain model mouse by using complete Freund's adjuvant (CFA); (2) increasing the expression quantity of the miRNA in spinal cord of the inflammation type chronic pain model mouse by using a miR-218-5p stimulant; (3) detecting the inhibition function of the miR-218-5p on SYNGR1 protein expression of CFA-resulted inflammation type chronic pain by using a Western blot method; and (4) detecting the expression of a spinal cord nerve cell activity marker c-fos of the miR-218-5p for inhibiting the inflammation type chronic pain state. The process is simple and low in cost, and the inflammation type chronic pain is effectively inhibited by using the miR-218-5p, and an effective interference target is provided for prevention and treatment on chronic pain. The further quantitative analysis on a blood clinical specimen of a patient suffering chronic pain shows that the miR-218-5p can be used as a marker of occurrence of chronic pain.

Description

technical field [0001] The present invention relates to the use of a microRNA molecule, in particular to the use of miRNA in inflammatory chronic pain, specifically the application of a miR-218-5p compound as a chronic pain marker and in the treatment of chronic pain drugs, belonging to The field of biomedical materials technology. Background technique [0002] Pain is one of the most common clinical symptoms. Long-term pathological pain accompanied by psychological and emotional diseases has brought unbearable torture to the lives of chronic pain patients. According to statistics, there are as many as 320 million chronic pain patients in the world. Governments around the world have invested a lot of manpower and hundreds of billions of dollars in financial resources to try to overcome this problem. In China alone, there are hundreds of millions of pain patients. Undoubtedly, it is a difficult problem that human beings can't wait to solve. [0003] Nerve cells contain more...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A61K48/00A61P25/04A61P29/00
CPCA61K48/005C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 曹君利李燕强杨俊霞
Owner XUZHOU MEDICAL COLLEGE
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